Nothing
# Load necessary libraries
library(dplyr)
library(tidyr)
library(ggplot2)
library(diceplot)
# Define data
cell_types <- c("TypeA", "TypeB", "TypeC")
pathways <- c("Pathway1", "Pathway2", "Pathway3")
pathway_groups <- data.frame(
Pathway = pathways,
Group = c("Group1", "Group2", "Group3"),
stringsAsFactors = FALSE
)
set.seed(123)
replicates <- 10
data <- expand.grid(
CellType = cell_types,
Pathway = pathways,
Replicate = 1:replicates,
stringsAsFactors = FALSE
)
data <- data %>%
mutate(
treat_time = sample(c("0", "2", "6", "18"), size = n(), replace = TRUE)
)
missing_treat_times <- setdiff(c("0", "2", "6", "18"), unique(data$treat_time))
if(length(missing_treat_times) > 0){
data$treat_time[1:length(missing_treat_times)] <- missing_treat_times
}
data <- data %>%
mutate(
treat_time = factor(treat_time, levels =c( "0","2","6","18"))
) %>%
left_join(pathway_groups, by = "Pathway") %>%
select(-Replicate)
# Create and plot the dice plot
p <- dice_plot(
data = data,
cat_a = "treat_time",
cat_b = "Pathway",
cat_c = "CellType",
group = "Group",
group_alpha = 0.6,
title = "Dice Plot with 6 Pathology Variables and Treatment Time",
custom_theme = theme_minimal(),
min_dot_size = 2,
max_dot_size = 4,
cluster_by_column = F,
cluster_by_row = FALSE,
)
print(p)
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