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freqpcr: Estimates Allele Frequency on qPCR DeltaDeltaCq from Bulk Samples
Interval estimation of the population allele frequency from qPCR analysis based on the restriction enzyme digestion (RED)-DeltaDeltaCq method (Osakabe et al. 2017, <doi:10.1016/j.pestbp.2017.04.003>), as well as general DeltaDeltaCq analysis. Compatible with the Cq measurement of DNA extracted from multiple individuals at once, so called "group-testing", this model assumes that the quantity of DNA extracted from an individual organism follows a gamma distribution. Therefore, the point estimate is robust regarding the uncertainty of the DNA yield.
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Package details |
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| Author | Masaaki Sudo [aut, cre] (<https://orcid.org/0000-0001-9834-9857>) |
| Maintainer | Masaaki Sudo <masaaki@sudori.info> |
| License | GPL (>= 3) |
| Version | 0.4.0 |
| URL | https://github.com/sudoms/freqpcr |
| Package repository | View on CRAN |
| Installation |
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