R/dataset-mcroy1970.R

#' Eelgrass phosphate incorporation data (McRoy & Barsdate 1970)
#'
#' Dataset built from the article "Phosphate absorption in eelgrass" by McRoy
#' and Barsdate (1970)
#'
#' In brief, the experimental setup consists in individual eelgrass plants
#' placed in 250 ml containers. Each container is partitioned by a layer of
#' paraffin into an upper water compartment (containing the leaves and stems)
#' and a lower water compartment (containing the roots and rhizomes).
#'
#' Radioactive phosphorus (32P) is added as phosphate either in the upper or
#' lower water compartment in each container. Containers were incubated either
#' in light or dark conditions.
#'
#' Tissue samples were collected and dried at various time points and 32P
#' activity was measured (Figure 2 in the original paper). Biomass estimates in
#' initial conditions were given in Table 1 of the original paper.
#' 
#' @section Data preparation:
#'
#' The data for 32P abundance per mg is extracted from Figure 2 of the original
#' article. Atom counts per mg were derived from cpm per mg using a half-life
#' value of 14.268 days for 32P.
#'
#' For simplicity and in order to be able to match the 32P data with the
#' biomass data (see below), only four compartments are considerd in the
#' package dataset. Upper and lower water compartments match the compartments
#' from the original article. "Leaf and stem" pools the original compartments
#' "leaf tip", "leaf middle", "leaf base", and "stem". "Roots and rhizome"
#' pools the original compartments "root" and "rhizome". Pooling is done by
#' averaging the cpm per mg data, thereby making the rough approximation that
#' each component of the pool contributes the same biomass as the other
#' components.
#'
#' The biomass data is taken from Table 1 in the original paper. Experimental
#' containers had 160 cc of seawater in the upper compartment and 80 cc of
#' seawater in the lower compartment. Based on comparison with data from
#' Risgaard-Petersen 1998, I assumed that the biomasses for tissues were given
#' in dry weight. I assumed that this was also the case for the cpm/mg data
#' (i.e. cpm/mg of dry weight).
#' 
#' @source Data was taken from the figures and tables of the original
#'     paper. The original paper is: McRoy, C. Peter, and Robert
#'     J. Barsdate. “Phosphate Absorption in Eelgrass1.” Limnology and
#'     Oceanography 15, no. 1 (January 1, 1970):
#'     6–13. https://doi.org/10.4319/lo.1970.15.1.0006.
#'
#' @format Tibble with columns
#' \describe{
#'   \item{light_treatment}{Light treatment: "light" or "dark".}
#'   \item{addition_site}{The location where 32P phosphate was added: in the
#'     "upper" water compartment or in the "lower" water compartment.}
#'   \item{compartment}{Obsered compartment, one of "leaves_stem",
#'     "roots_rhizome", "upper_water", or "lower_water".}
#'   \item{time_min}{Elapsed time in minutes since the 32P addition.}
#'   \item{n_32P_per_mg}{Number of 32P atoms per mg (estimated from Figure 2 of
#'     the original paper).}
#'   \item{mass_mg}{Compartment mass in mg (taken from Table 1 of the original
#'     paper). Assumed constant during the experiment.}
#'   \item{n_32P}{Number of 32P atoms in the compartment. Calculated from the
#'     two previous columns.}
#' }

"eelgrass"

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isotracer documentation built on Sept. 22, 2023, 1:07 a.m.