jackstraw_subspace: Jackstraw for the User-Defined Dimension Reduction Methods

In jackstraw: Statistical Inference for Unsupervised Learning

Jackstraw for the User-Defined Dimension Reduction Methods

Description

Test association between the observed variables and their latent variables, captured by a user-defined dimension reduction method.

Usage

jackstraw_subspace(
  dat,
  r,
  FUN,
  r1 = NULL,
  s = NULL,
  B = NULL,
  covariate = NULL,
  noise = NULL,
  verbose = TRUE
)

Arguments

dat	a data matrix with m rows as variables and n columns as observations.
r	a number of significant latent variables.
FUN	Provide a specific function to estimate LVs. Must output r estimated LVs in a n*r matrix.
r1	a numeric vector of latent variables of interest.
s	a number of “synthetic” null variables. Out of m variables, s variables are independently permuted.
B	a number of resampling iterations.
covariate	a model matrix of covariates with n observations. Must include an intercept in the first column.
noise	specify a parametric distribution to generate a noise term. If NULL, a non-parametric jackstraw test is performed.
verbose	a logical specifying to print the computational progress.

Details

This function computes m p-values of linear association between m variables and their latent variables, captured by a user-defined dimension reduction method. Its resampling strategy accounts for the over-fitting characteristics due to direct computation of PCs from the observed data and protects against an anti-conservative bias.

This function allows you to specify a parametric distribution of a noise term. It is an experimental feature. Then, a small number s of observed variables are replaced by synthetic null variables generated from a specified distribution.

Value

jackstraw_subspace returns a list consisting of

p.value	m p-values of association tests between variables and their principal components
obs.stat	m observed statistics
null.stat	s*B null statistics

Author(s)

Neo Christopher Chung [e-mail]

References

Chung and Storey (2015) Statistical significance of variables driving systematic variation in high-dimensional data. Bioinformatics, 31(4): 545-554 https://academic.oup.com/bioinformatics/article/31/4/545/2748186

Chung (2020) Statistical significance of cluster membership for unsupervised evaluation of cell identities. Bioinformatics, 36(10): 3107–3114 https://academic.oup.com/bioinformatics/article/36/10/3107/5788523

See Also

jackstraw_pca jackstraw

Examples

## simulate data from a latent variable model: Y = BL + E
B = c(rep(1,50),rep(-1,50), rep(0,900))
L = rnorm(20)
E = matrix(rnorm(1000*20), nrow=1000)
dat = B %*% t(L) + E
dat = t(scale(t(dat), center=TRUE, scale=TRUE))

## apply the jackstraw with the svd as a function
out = jackstraw_subspace(dat, FUN = function(x) svd(x)$v[,1,drop=FALSE], r=1, s=100, B=50)

jackstraw documentation built on Nov. 25, 2022, 5:06 p.m.