normalize_sum: Normalize intensities across samples by dividing by the...

In metamorphr: Tidy and Streamlined Metabolomics Data Workflows

Normalize intensities across samples by dividing by the sample sum

Description

Normalize across samples by dividing feature intensities by the sum of all intensities in a sample, making the sum 1 in all samples.

Important Note

Intensities of individual features will be very small after this normalization approach. It is therefore advised to multiply all intensities with a fixed number (e.g., 1000) after normalization. See this discussion on OMICSForum.ca and the examples below for further information.

Usage

normalize_sum(data)

Arguments

data	A tidy tibble created by read_featuretable.

Value

A tibble with intensities normalized across samples.

Examples

# Example 1: Normalization only
toy_metaboscape %>%
  normalize_sum()

# Example 2: Multiply with 1000 after normalization
toy_metaboscape %>%
  normalize_sum() %>%
  dplyr::mutate(Intensity = .data$Intensity * 1000)

metamorphr documentation built on June 10, 2026, 5:07 p.m.