calculateAT6: Calculate Analytical Threshold

Description Usage Arguments Details Value References See Also

View source: R/calculateAT6.r


Calculate analytical thresholds estimate using linear regression.


calculateAT6(data, ref, amount = NULL, weighted = TRUE, alpha = 0.05, = TRUE, debug = FALSE)



data.frame containing at least columns 'Sample.Name', 'Marker', 'Allele', and 'Height'.


data.frame containing at least columns 'Sample.Name', 'Marker', and 'Allele'.


data.frame containing at least columns 'Sample.Name' and 'Amount'. If NULL 'data' must contain a column 'Amount'.


logical to calculate weighted linear regression (weight=1/se^2).


numeric [0,1] significance level for the t-statistic.

logical to indicate if sample matching should ignore case.


logical to indicate if debug information should be printed.


Calculate the analytical threshold (AT) according to method 6 as outlined in the reference. In short serial dilutions are analyzed and the average peak height is calculated. Linear regression or Weighted linear regression with amount of DNA as the predictor for the peak height is performed. Method 6: A simplified version of the upper limit approach. AT6 = y-intercept + t-statistic * standard error of the regression. Assumes the y-intercept is not different from the mean blank signal. The mean blank signal should be included in the confidence range ('Lower' to 'AT6' in the resulting data frame). NB! This is an indirect method to estimate AT and should be verified by other methods. From the reference: A way to determine the validity of this approach is based on whether the y-intercept +- (1-a)100 contains the mean blank signal. If the mean blank signal is included in the y-intercept band, the following relationship [i.e. AT6] can be used to determine the AT. However, it should be noted that the ATs derived in this manner need to be calculated for each color and for all preparations (i.e., different injections, sample preparation volumes, post-PCR cleanup, etc.). NB! Quality sensors must be removed prior to analysis.


data.frame with columns 'Amount', 'Height', 'Sd', 'Weight', 'N', 'Alpha', 'Lower', 'Intercept', and 'AT6'.


J. Bregu, Analytical thresholds and sensitivity: establishing RFU thresholds for forensic DNA analysis, J. Forensic Sci. 58 (1) (2013) 120-129, ISSN 1556-4029, DOI: 10.1111/1556-4029.12008.

See Also

calculateAT6_gui, calculateAT, calculateAT_gui, lm

strvalidator documentation built on Aug. 12, 2017, 1:03 a.m.