greenThresh: Thresholds a 'Frames' object for green pixels

Description Usage Arguments Value Author(s) Examples

Description

Green pixels, or pixels with H value between 61/360 and 140/360 and S value over 0.15 in the HSV color scheme, are treated as foreground. All other pixels are treated as background. This is useful for detecting the Green Flourescence Protein used to denote that a cell is alive.

Usage

1
greenThresh(frames)

Arguments

frames

A Frames object with all 3 color channels(R,G,B)

Value

a binary Frames object in the green color channel

Author(s)

Ayan Bandyopadhyay, Bellarmine College Prep 11/26/2015

Examples

1
2
library(flowcatchR)
threshedFrames <- greenThresh(MesenteriumSubset)

Ayan1/Cell-Trajectory-Analysis documentation built on May 5, 2019, 9:23 a.m.