Description Usage Arguments Value Author(s)
It normalises single cells from countsRaw slot within the
RNAseq
object provided. It follows Brennecke et al., Nature Methods, 2013.
1 2 3 4 5 6 |
object |
|
cells |
vector of cells to normalise. Default: NULL. This will first look at the
|
genes |
vector of genes to normalise. Default: NULL (It will take all the genes of
the |
genes.SizF |
Genes to use to compute genes size factors for each cell. Default: Vector of "genes" that are not ERCCs. |
ercc.SizF |
ERCCs to use to compute ERCC size factors for each cell. Default: Vector of "erccs". |
List with normalised counts Matrix (countsNorm) and size factors for biological
genes (sizeFactorsGenes) and spike-ins (sizeFactorsSpikeIn). countsNorm should be stored in
object@countsNorm
slot and the size factors should be stored in
object@CellsSizeFac
slot as a list (with the two types of size factors).
Blanca Pijuan Sala.
Add the following code to your website.
For more information on customizing the embed code, read Embedding Snippets.