Dataset used in Cursons et al. 2015. where parallel ‘deep sequencing’ of RNA (RNA-Seq) were conducted to examine the changes in expression profiles between breast cancer cell lines PMC42-ET, PMC42-LA and MDA-MB-468 cells.
A SummarizedExperiment object, containing gene expression data of
different sub-lines of human breast cancer cell lines. The
SummarizedExperiment::colData() function can be used to access the sample
For PMC42 cell lines, cells were treated for 3 or 7 days in the presence or absence of 10 ng/ml EGF. For MDA-MB-468 cells, cells were treated for 7 days in the presence or absence of either 10 ng/ml EGF or kept under Hypoxia.
There are 3 biological replicates per condition which were summed. Samples were sequenced on the Illumina HiSeq 2000, 100bp paired end.
Data from this publication were downloaded from EMBL-EBI ENA and processed into a SummarizedExperiment object. Sample annotations were modified from the original publication and SRA portal.
Cursons, J., Leuchowius, KJ., Waltham, M., Tomaskovic-Crook, E., Foroutan, M., Bracken, CP., Redfern,A., Crampin, EJ., Street, I., Davis, MJ. & Thompson,EW. (2015) Stimulus-dependent differences in signalling regulate epithelial-mesenchymal plasticity and change the effects of drugs in breast cancer cell lines. Cell Commun Signal 13, 26 (2015).
1 2 3
library(ExperimentHub) eh <- ExperimentHub() emt_datasets <- query(eh, "emtdata")
Add the following code to your website.
For more information on customizing the embed code, read Embedding Snippets.