ligation: Ligation Calculator

In JulianSpagnuolo/Bioscikit: Biotools

Description

Calculates the mass of insert fragment required for a ligation reaction.

Usage

ligation(molar.ratio, vector.mass, vector.size, insert.size)

Arguments

molar.ratio	Molar ratio of insert to vector
vector.mass	mass of vector fragment used in ligation reaction, usually in ng
vector.size	size of vector fragment to ligate in kilo base pairs
insert.size	size of insert fragment to ligate in kilo base pairs

Details

returns the mass of the insert fragment in the same units as the vector mass, usually ng.

Value

numeric value corresponding to the mass of the insert fragment in the same units as the vector mass, usually ng.

Author(s)

Julian Spagnuolo

Examples

## Calculating a ligation of a 1 kBP insert to 180 ng of a 2.7 kBP vector using insert-to-vector ratio of 2:1

ligation(molar.ratio = 2/1, vector.mass = 180, vector.size = 2.7, insert.size = 1)

JulianSpagnuolo/Bioscikit documentation built on May 28, 2019, 8:22 a.m.