pcr: PCR Setup Calculator
In JulianSpagnuolo/Bioscikit: Biotools

Description Usage Arguments Value Author(s) Examples

Calculates the volumes of stock PCR reagents required for a single template

pcr(stock.fwd.prim, stock.rev.prim, stock.dNTP, stock.PCR.buffer, stock.DNApol, final.fwd.prim = 0.2, final.rev.prim = 0.2, final.dNTP, final.PCR.buffer = 1, final.DNApol, stock.template, final.template, rxn.vol = 50)

`stock.fwd.prim`	Stock concentration of forward primer, usually in micro moles per Litre
`stock.rev.prim`	Stock concentration of reverse primer, usually in micro moles per Litre
`stock.dNTP`	Stock concentration of dNTP mix, can be in micromoles per litre or an arbitrary concentration like 100x
`stock.PCR.buffer`	Stock concentration of PCR buffer
`stock.DNApol`	Stock concentration of DNA polymerase, can be in units per microLitre or an arbitrary concentration like 100x
`stock.template`	Initial concentration of template DNA, usually in ng per microlitre
`final.fwd.prim`	Desired concentration of forward primer in final PCR volume, defualt is 200 nM
`final.rev.prim`	Desired concentration of reverse primer in final PCR volume, defualt is 200 nM
`final.dNTP`	Desired concentration of dNTPs in the final PCR volume
`final.PCR.buffer`	Desired concentration of PCR Buffer in the final PCR volume, usually 1x
`final.DNApol`	Desired final concentration of DNA polymerase in the PCR reaction
`final.template`	Desired final concentration of template DNA in the PCR reaction
`rxn.vol`	Desired total volume of the PCR reaction, usually 50 microlitres

Returns a data.frame of values corresponding to the volumes of stock solutions required.

Julian Spagnuolo

pcr(stock.fwd.prim = 6.25, stock.rev.prim = 6.25, stock.dNTP = 10, stock.PCR.buffer = 10, stock.DNApol = 50, stock.template = 20, final.fwd.prim = 0.2, final.rev.prim =0.2 , final.dNTP = 1, final.PCR.buffer = 1, final.DNApol = 1, final.template = 0.1, rxn.vol = 50)