Description Usage Arguments Value Author(s) Examples
Calculates the volumes of stock PCR reagents required for a single template
1 | pcr(stock.fwd.prim, stock.rev.prim, stock.dNTP, stock.PCR.buffer, stock.DNApol, final.fwd.prim = 0.2, final.rev.prim = 0.2, final.dNTP, final.PCR.buffer = 1, final.DNApol, stock.template, final.template, rxn.vol = 50)
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stock.fwd.prim |
Stock concentration of forward primer, usually in micro moles per Litre |
stock.rev.prim |
Stock concentration of reverse primer, usually in micro moles per Litre |
stock.dNTP |
Stock concentration of dNTP mix, can be in micromoles per litre or an arbitrary concentration like 100x |
stock.PCR.buffer |
Stock concentration of PCR buffer |
stock.DNApol |
Stock concentration of DNA polymerase, can be in units per microLitre or an arbitrary concentration like 100x |
stock.template |
Initial concentration of template DNA, usually in ng per microlitre |
final.fwd.prim |
Desired concentration of forward primer in final PCR volume, defualt is 200 nM |
final.rev.prim |
Desired concentration of reverse primer in final PCR volume, defualt is 200 nM |
final.dNTP |
Desired concentration of dNTPs in the final PCR volume |
final.PCR.buffer |
Desired concentration of PCR Buffer in the final PCR volume, usually 1x |
final.DNApol |
Desired final concentration of DNA polymerase in the PCR reaction |
final.template |
Desired final concentration of template DNA in the PCR reaction |
rxn.vol |
Desired total volume of the PCR reaction, usually 50 microlitres |
Returns a data.frame of values corresponding to the volumes of stock solutions required.
Julian Spagnuolo
1 | pcr(stock.fwd.prim = 6.25, stock.rev.prim = 6.25, stock.dNTP = 10, stock.PCR.buffer = 10, stock.DNApol = 50, stock.template = 20, final.fwd.prim = 0.2, final.rev.prim =0.2 , final.dNTP = 1, final.PCR.buffer = 1, final.DNApol = 1, final.template = 0.1, rxn.vol = 50)
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