pcr_lib_qc: Create library prep quality control data

In KaiAragaki/amplify: Automate PCR Tasks Reproducibly

Create library prep quality control data

Description

Create library prep quality control data

Usage

pcr_lib_qc(lib_calc_pcr)

Arguments

lib_calc_pcr

A pcr object, output from pcr_lib_calc

Details

While the output of this function on its own is can theoretically be used to gauge library quality, it is best used in conjunction with a function like pcr_lib_calc_report

Value

a pcr object with list with:

• standards Data for individual standards, including calculated dilutions, given and calculated quantities, raw Ct, etc.

• samples Data for individual samples, including calculated concentrations, raw Ct, etc.

• sample_summary Summary statistics for samples grouped by replicates

• standard_summary Summary statistics for standards groupd by replicates

• outliers Data for individual samples and standards with and without their putative outliers (po) per replicate group

Examples

system.file("extdata", "untidy-standard-curve.xlsx", package = "amplify") |>
  pcr_tidy(pad_zero = TRUE) |>
  pcr_lib_calc() |>
  pcr_lib_qc()

KaiAragaki/amplify documentation built on Oct. 14, 2024, 11:46 p.m.