plot_run_discovery_analysis: Plot run discovery analysis

In Katsevich-Lab/sceptre: Analysis of Single-Cell CRISPR Screen Data

Plot run discovery analysis

Description

plot_run_discovery_analysis() creates a visualization of the outcome of the discovery analysis. The visualization consists of four plots:

• The upper left plot superimposes the discovery p-values (blue) on top of the negative control p-values (red) on an untransformed scale.

• The upper right plot is the same as the upper left plot, but the scale is negative log-10 transformed. The discovery p-values ideally should trend above the diagonal line, indicating the presence of signal in the discovery set. The horizontal dashed line indicates the multiple testing threshold; discovery pairs whose p-value falls above this line are called as significant.

• The bottom left panel is a volcano plot of the p-values and log fold changes of the discovery pairs. Each point corresponds to a pair; the estimated log-2 fold change of the pair is plotted on the horizontal axis, and the (negative log-10 transformed) p-value is plotted on the vertical axis. The horizontal dashed line again indicates the multiple testing threshold. Points above the dashed line (colored in purple) are called as discoveries, while points below (colored in blue) are called as insignificant.

• The bottom right panel is a text box displaying the number of discovery pairs called as significant.

Usage

plot_run_discovery_analysis(
  sceptre_object,
  x_limits = c(-1.5, 1.5),
  point_size = 0.55,
  transparency = 0.8,
  return_indiv_plots = FALSE
)

Arguments

sceptre_object	a sceptre_object that has had run_discovery_analysis called on it
x_limits	(optional; default c(-1.5, 1.5)) a numeric vector of length 2 giving the lower and upper limits of the x-axis (corresponding to log-2 fold change) for the "Discovery volcano plot" panel
point_size	(optional; default 0.55) the size of the individual points in the plot
transparency	(optional; default 0.8) the transparency of the individual points in the plot
return_indiv_plots	(optional; default FALSE) if FALSE then a list of ggplot is returned; if TRUE then a single cowplot object is returned.

Value

a single cowplot object containing the combined panels (if return_indiv_plots is set to TRUE) or a list of the individual panels (if return_indiv_plots is set to FALSE)

Examples

library(sceptredata)
data(highmoi_example_data)
data(grna_target_data_frame_highmoi)
# import data
sceptre_object <- import_data(
  response_matrix = highmoi_example_data$response_matrix,
  grna_matrix = highmoi_example_data$grna_matrix,
  grna_target_data_frame = grna_target_data_frame_highmoi,
  moi = "high",
  extra_covariates = highmoi_example_data$extra_covariates,
  response_names = highmoi_example_data$gene_names
)
positive_control_pairs <- construct_positive_control_pairs(sceptre_object)
discovery_pairs <- construct_cis_pairs(sceptre_object,
  positive_control_pairs = positive_control_pairs,
  distance_threshold = 5e6
)
sceptre_object |>
  set_analysis_parameters(
    side = "left",
    discovery_pairs = discovery_pairs,
    resampling_mechanism = "permutations",
  ) |>
  assign_grnas(method = "thresholding") |>
  run_qc() |>
  run_calibration_check(
    parallel = TRUE,
    n_processors = 2
  ) |>
  run_discovery_analysis(
    parallel = TRUE,
    n_processors = 2
  ) |>
  plot_run_discovery_analysis()

Katsevich-Lab/sceptre documentation built on Nov. 19, 2024, 5:16 p.m.