calc.Cq.qpcR: calculates Cq values

In LucasFVoges/DoubleqpcR: DMAS-qPCR data analysis

calculates Cq values

Description

WRAPPER for qpcR Package 'qpcR: Modelling and Analysis of Real-Time PCR Data, Andrej-Nikolai Spiess, 2018'

Usage

calc.Cq.qpcR(method = "TP", fct = l4, cq.new = "calc.qpcR.Cq")

Arguments

method	The method used to calculate the Cq value. "TP / cpD1" for Turning Point (first derivative) . "SD / cpD2" for first exponential incline (second derivative). "cpE", "cpR", "cpT", "Cy0", "cpCQ", "cpMR" (see qpcR package)
fct	will be used by qpcR as fitting function. l4, l5, b4
cq.new	The Column name of the new Cq values.

Details

This function will calculate Cq values for the data frame input.cq You need to have input.cq and input.raw from "read.fluorescenceTable" / "read.cqTable" or build them manually.

Please note, that it is faster to use qpcR directly!

Value

does not return! adds Cq value to input.cq data frame

LucasFVoges/DoubleqpcR documentation built on Feb. 19, 2024, 7:21 p.m.