FragPipetoMSstatsFormat: Import FragPipe files

View source: R/converters.R

FragPipetoMSstatsFormatR Documentation

Import FragPipe files

Description

Import FragPipe files

Usage

FragPipetoMSstatsFormat(
  input,
  useUniquePeptide = TRUE,
  removeFewMeasurements = TRUE,
  removeProtein_with1Feature = FALSE,
  summaryforMultipleRows = max,
  use_log_file = TRUE,
  append = FALSE,
  verbose = TRUE,
  log_file_path = NULL,
  ...
)

Arguments

input

name of FragPipe msstats.csv export. ProteinName, PeptideSequence, PrecursorCharge, FragmentIon, ProductCharge, IsotopeLabelType, Condition, BioReplicate, Run, Intensity are required.

useUniquePeptide

TRUE (default) removes peptides that are assigned for more than one proteins. We assume to use unique peptide for each protein.

removeFewMeasurements

TRUE (default) will remove the features that have 1 or 2 measurements across runs.

removeProtein_with1Feature

TRUE will remove the proteins which have only 1 feature, which is the combination of peptide, precursor charge, fragment and charge. FALSE is default.

summaryforMultipleRows

max(default) or sum - when there are multiple measurements for certain feature and certain run, use highest or sum of multiple intensities.

use_log_file

logical. If TRUE, information about data processing will be saved to a file.

append

logical. If TRUE, information about data processing will be added to an existing log file.

verbose

logical. If TRUE, information about data processing wil be printed to the console.

log_file_path

character. Path to a file to which information about data processing will be saved. If not provided, such a file will be created automatically. If 'append = TRUE', has to be a valid path to a file.

...

additional parameters to 'data.table::fread'.

Value

data.frame in the MSstats required format.

Author(s)

Devon Kohler

Examples

fragpipe_raw = system.file("tinytest/raw_data/FragPipe/fragpipe_input.csv",
                              package = "MSstatsConvert")
fragpipe_raw = data.table::fread(fragpipe_raw)
fragpipe_imported = FragPipetoMSstatsFormat(fragpipe_raw, use_log_file = FALSE)
head(fragpipe_imported)


MeenaChoi/MSstats documentation built on July 13, 2024, 10:59 a.m.