ProgenesistoMSstatsFormat: Import Progenesis files

View source: R/converters.R

ProgenesistoMSstatsFormatR Documentation

Import Progenesis files

Description

Import Progenesis files

Usage

ProgenesistoMSstatsFormat(
  input,
  annotation,
  useUniquePeptide = TRUE,
  summaryforMultipleRows = max,
  removeFewMeasurements = TRUE,
  removeOxidationMpeptides = FALSE,
  removeProtein_with1Peptide = FALSE,
  use_log_file = TRUE,
  append = FALSE,
  verbose = TRUE,
  log_file_path = NULL,
  ...
)

Arguments

input

name of Progenesis output, which is wide-format. 'Accession', 'Sequence', 'Modification', 'Charge' and one column for each run are required.

annotation

name of 'annotation.txt' or 'annotation.csv' data which includes Condition, BioReplicate, Run information. It will be matched with the column name of input for MS runs.

useUniquePeptide

TRUE (default) removes peptides that are assigned for more than one proteins. We assume to use unique peptide for each protein.

summaryforMultipleRows

max(default) or sum - when there are multiple measurements for certain feature and certain run, use highest or sum of multiple intensities.

removeFewMeasurements

TRUE (default) will remove the features that have 1 or 2 measurements across runs.

removeOxidationMpeptides

TRUE will remove the peptides including 'oxidation (M)' in modification. FALSE is default.

removeProtein_with1Peptide

TRUE will remove the proteins which have only 1 peptide and charge. FALSE is default.

use_log_file

logical. If TRUE, information about data processing will be saved to a file.

append

logical. If TRUE, information about data processing will be added to an existing log file.

verbose

logical. If TRUE, information about data processing wil be printed to the console.

log_file_path

character. Path to a file to which information about data processing will be saved. If not provided, such a file will be created automatically. If 'append = TRUE', has to be a valid path to a file.

...

additional parameters to 'data.table::fread'.

Value

data.frame in the MSstats required format.

Author(s)

Meena Choi, Olga Vitek, Ulrich Omasits

Examples

progenesis_raw = system.file("tinytest/raw_data/Progenesis/progenesis_input.csv", 
                             package = "MSstatsConvert")
annot = system.file("tinytest/raw_data/Progenesis/progenesis_annot.csv", 
                    package = "MSstatsConvert")
progenesis_raw = data.table::fread(progenesis_raw) 
annot = data.table::fread(annot)

progenesis_imported = ProgenesistoMSstatsFormat(progenesis_raw, annot,
                                                use_log_file = FALSE)
head(progenesis_imported)


MeenaChoi/MSstats documentation built on July 13, 2024, 10:59 a.m.