flowDensity-methods: Methods for Function 'flowDensity' in Package 'flowDensity'

In RGLab/flowDensity: Sequential Flow Cytometry Data Gating

Description

Methods for function flowDensity in package flowDensity

Usage

## S4 method for signature 'CellPopulation,ANY,logical,missing'
flowDensity(obj, channels, position,node, ...)
## S4 method for signature 'flowFrame,ANY,logical,missing'
flowDensity(obj, channels, position,node, ...)
## S4 method for signature 'GatingHierarchy,ANY,logical,ANY'
flowDensity(obj, channels, position,node, ...)

Arguments

obj	GatingHierarchy or CellPopulationobject
channels	a vector of two channel names or their corresponding indices.
position	a vector of two logical values specifying the position of the cell subset of interest on the 2D plot.
node	A character defining the parent population when extracting data from GatingHierarchy.
...	This can be used to pass one of the following arguments: 'use.percentile' if TRUE, returns the 'percentile'th threshold. 'percentile' a value in [0,1] that is used as the percentile if 'use.percentile' is TRUE. 'upper' if 'TRUE', it finds the change in the slope after the peak with index 'peak.ind'. 'use.upper' if 'TRUE', forces to return the inflection point based on the first (last) peak if upper=F (upper=T) 'twin.factor' a value in [0,1] that is used to exclude twinpeaks. 'bimodal' If TRUE, it returns a cutoff that splits population closer to 50-50, when there are more than two peaks. 'after.peak' If TRUE, it returns a cutoff that is after the maximum peaks, when there are more than two peaks. 'sd.threshold' if TRUE, it uses 'n.sd' times standard deviation for gating. 'n.sd' an integer that is multiplied to the standard deviation to determine the place of threshold if 'sd.threshold' is 'TRUE'. 'tinypeak.removal' a vector of length 2, for sensitivity of peak finding for each channel. See deGate() for more information. 'filter' If provided it uses the given filter to gate the population. 'use.control' if TRUE, it finds the threshold using a matched control population and uses it for gating. 'control' a 'flowFrame' or 'CellPopulation' object used for calculating the gating threshold when 'use.control' is set to TRUE. If a control population is used, the other arguments ('upper', 'percentile', etc.) are applied to the control data when finding the threshold (i.e. not to 'obj'). 'alpha' a value in [0,1) specifying the significance of change in the slope which would be detected. This is by default 0.1, and typically need not be changed. 'ellip.gate' if TRUE, it fits an ellipse on the data as a gate, otherwise the rectangle gating results are returned 'scale' a value in [0,1) that scales the size of ellipse to fit if 'ellip.gate' is TRUE

Value

a CellPopulation object.

RGLab/flowDensity documentation built on Sept. 12, 2019, 6:47 a.m.