README.md

In VallinP/easyFlow: easyFlow : An easy to do unsupervised analysis of mass and flow cytometry data

easyFlow

easyFlow : An easy to do unsupervised analysis for mass and flow cytometry data. From fcs data to statistics !

How To easyflow

Creation of a Virtual Machine (VM)

The complete easyFlow pipeline was tested on Linux Ubuntu operating system only (16.04 LTS 64bit).

To create a VM running on Ubuntu, please follow the link :

https://github.com/VallinP/HowTo_VirtualMachine

Install easyFlow package

devtools::install_github("VallinP/easyFlow")

easyFlow analysis

Before to start, you have to perform a quick analysis of your data to : - Compensate your data - Select events of interrest (ie, live cells, CD45+ ....) - Save the workspace in the same folder of your fcs.

Now, you are ready to start an easyFlow analysis.

1- Initialization

library(easyflow)

easyFlow_initialization()

2- Setup parameters

3- Analysis

4- Finalizing analysis

5- Interpretation

VallinP/easyFlow documentation built on Dec. 18, 2021, 6:14 p.m.