fpkm_to_counts: Turn FPKMs from a ballgown object into estimated counts for...
In alyssafrazee/polyester-release: Simulate RNA-seq reads

Turn FPKMs from a ballgown object into estimated counts for transcripts

1	fpkm_to_counts(bg, mean_rps = 1e+08, threshold = 0)

`bg`	ballgown object created from real RNA-seq dataset
`mean_rps`	This should be the number of reads per sample in total for use in backing out the FPKM calculations
`threshold`	only estimate parameters from transcripts with mean FPKM measurements larger than `threshold`

A matrix of counts with the same number of rows and columns as the ballgown object

Jeff Leek

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library(ballgown)
  data(bg)
  countmat = fpkm_to_counts(bg, mean_rps=400000)

alyssafrazee/polyester-release documentation built on May 12, 2019, 2:32 a.m.

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