brtSetNoiseBc: brtSetNoiseBc Estimate the noise level of bcs

In c57bl/Brt:

brtSetNoiseBc Estimate the noise level of bcs

Description

The data is noisy, it is important to identify noise. RV seldom infect non-neuronal cells from neurons, thus the bcs expressing levels of non-neuronal groups reflects the noise level of experiment. By measure the median + threshold * IQR, the threshold of signal can be determined.

Usage

brtSetNoiseBc(obj, background = NULL, threshold = 3, signal = "Neurons")

Arguments

obj	an brtstarter object, the idents of coldata should contain Neurons and at least one non-neuronal ident.
background	a character vector, describe which non-neuronal cluster used to calculate the threshold. Using all non-neuronal groups if none is provided.
threshold	a numeric value, default to be 3
signal	a character, describe which ident is signal, default is Neurons

c57bl/Brt documentation built on May 20, 2022, 8:45 p.m.