sigmoid: pHluorin Calibration Curve Analysis
In ctriandafillou/cat.extras:
Description
Usage
Arguments
Value
Description
This function takes raw data from a calibration curve experiment (yeast expressing pHluorin, flow cytometry data) and does all processing steps to construct a calibration curve that maps fluorescence ratio to pH. Takes *raw* (untransformed) FITC and BV510 channels as inputs!
Usage
1
sigmoid(params, x)
Arguments
params
character vector of parameters c(a, b, c, d); a is the max value, b is the steepness, c is the location of the inflection point, and d is the min value
x
x values to generate the sigmoid for
Value
vector of sigmoid function values for each x point given
ctriandafillou/cat.extras documentation built on Aug. 27, 2020, 11:04 p.m.
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Description Usage Arguments Value
Description
This function takes raw data from a calibration curve experiment (yeast expressing pHluorin, flow cytometry data) and does all processing steps to construct a calibration curve that maps fluorescence ratio to pH. Takes *raw* (untransformed) FITC and BV510 channels as inputs!
Usage
1 | sigmoid(params, x)
|
Arguments
params |
character vector of parameters c(a, b, c, d); a is the max value, b is the steepness, c is the location of the inflection point, and d is the min value |
x |
x values to generate the sigmoid for |
Value
vector of sigmoid function values for each x point given
R Package Documentation
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Note that we can't provide technical support on individual packages. You should contact the package authors for that.
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