sigmoid: pHluorin Calibration Curve Analysis

Description Usage Arguments Value

View source: R/sigmoid.R

Description

This function takes raw data from a calibration curve experiment (yeast expressing pHluorin, flow cytometry data) and does all processing steps to construct a calibration curve that maps fluorescence ratio to pH. Takes *raw* (untransformed) FITC and BV510 channels as inputs!

Usage

1
sigmoid(params, x)

Arguments

params

character vector of parameters c(a, b, c, d); a is the max value, b is the steepness, c is the location of the inflection point, and d is the min value

x

x values to generate the sigmoid for

Value

vector of sigmoid function values for each x point given


ctriandafillou/cat.extras documentation built on Aug. 27, 2020, 11:04 p.m.