dataSummarizationPTM: Process MS PTM and global protein data

Description Usage Arguments Value Examples

View source: R/dataSummarizationPTM.R

Description

Utilizes functionality from MSstats to clean, summarize, and normalize PTM and protein level data. Imputes missing values, protein and PTM level summarization from peptide level quantification. Applies global median normalization on peptide level data and normalizes between runs.

Usage

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dataSummarizationPTM(
  data,
  logTrans = 2,
  normalization = "equalizeMedians",
  normalization.PTM = "equalizeMedians",
  nameStandards = NULL,
  nameStandards.PTM = NULL,
  featureSubset = "all",
  featureSubset.PTM = "all",
  remove_uninformative_feature_outlier = FALSE,
  remove_uninformative_feature_outlier.PTM = FALSE,
  min_feature_count = 2,
  min_feature_count.PTM = 1,
  n_top_feature = 3,
  n_top_feature.PTM = 3,
  summaryMethod = "TMP",
  equalFeatureVar = TRUE,
  censoredInt = "NA",
  MBimpute = TRUE,
  MBimpute.PTM = TRUE,
  remove50missing = FALSE,
  fix_missing = NULL,
  maxQuantileforCensored = 0.999,
  use_log_file = TRUE,
  append = TRUE,
  verbose = TRUE,
  log_file_path = NULL,
  base = "MSstatsPTM_log_"
)

Arguments

data

name of the list with PTM and (optionally) Protein data.tables, which can be the output of the MSstatsPTM converter functions

logTrans

logarithm transformation with base 2(default) or 10

normalization

normalization for the protein level dataset, to remove systematic bias between MS runs. There are three different normalizations supported. 'equalizeMedians'(default) represents constant normalization (equalizing the medians) based on reference signals is performed. 'quantile' represents quantile normalization based on reference signals is performed. 'globalStandards' represents normalization with global standards proteins. FALSE represents no normalization is performed

normalization.PTM

normalization for PTM level dataset. Default is "equalizeMedians" Can be adjusted to any of the options described above.

nameStandards

vector of global standard peptide names for protein dataset. only for normalization with global standard peptides.

nameStandards.PTM

Same as above for PTM dataset. "all"(default) uses all features that the data set has. "top3" uses top 3 features which have highest average of log2(intensity) across runs. "topN" uses top N features which has highest average of log2(intensity) across runs. It needs the input for n_top_feature option. "highQuality" flags uninformative feature and outliers

featureSubset

"all" (default) uses all features that the data set has. "top3" uses top 3 features which have highest average of log-intensity across runs. "topN" uses top N features which has highest average of log-intensity across runs. It needs the input for n_top_feature option. "highQuality" flags uninformative feature and outliers.

featureSubset.PTM

For PTM dataset only. Options same as above.

remove_uninformative_feature_outlier

For protein dataset only. It only works after users used featureSubset="highQuality" in dataProcess. TRUE allows to remove 1) the features are flagged in the column, feature_quality="Uninformative" which are features with bad quality, 2) outliers that are flagged in the column, is_outlier=TRUE, for run-level summarization. FALSE (default) uses all features and intensities for run-level summarization.

remove_uninformative_feature_outlier.PTM

For PTM dataset only. Options same as above.

min_feature_count

optional. Only required if featureSubset = "highQuality". Defines a minimum number of informative features a protein needs to be considered in the feature selection algorithm.

min_feature_count.PTM

For PTM dataset only. Options the same as above. Default is 1 due to low average feature count for PTMs.

n_top_feature

For protein dataset only. The number of top features for featureSubset='topN'. Default is 3, which means to use top 3 features.

n_top_feature.PTM

For PTM dataset only. Options same as above.

summaryMethod

"TMP"(default) means Tukey's median polish, which is robust estimation method. "linear" uses linear mixed model.

equalFeatureVar

only for summaryMethod="linear". default is TRUE. Logical variable for whether the model should account for heterogeneous variation among intensities from different features. Default is TRUE, which assume equal variance among intensities from features. FALSE means that we cannot assume equal variance among intensities from features, then we will account for heterogeneous variation from different features.

censoredInt

Missing values are censored or at random. 'NA' (default) assumes that all 'NA's in 'Intensity' column are censored. '0' uses zero intensities as censored intensity. In this case, NA intensities are missing at random. The output from Skyline should use '0'. Null assumes that all NA intensites are randomly missing.

MBimpute

For protein dataset only. only for summaryMethod="TMP" and censoredInt='NA' or '0'. TRUE (default) imputes 'NA' or '0' (depending on censoredInt option) by Accelated failure model. FALSE uses the values assigned by cutoffCensored.

MBimpute.PTM

For PTM dataset only. Options same as above.

remove50missing

only for summaryMethod="TMP". TRUE removes the runs which have more than 50% missing values. FALSE is default.

fix_missing

Default is Null. Optional, same as the 'fix_missing' parameter in MSstatsConvert::MSstatsBalancedDesign function

maxQuantileforCensored

Maximum quantile for deciding censored missing values. default is 0.999

use_log_file

logical. If TRUE, information about data processing will be saved to a file.

append

logical. If TRUE, information about data processing will be added to an existing log file.

verbose

logical. If TRUE, information about data processing will be printed to the console.

log_file_path

character. Path to a file to which information about data processing will be saved. If not provided, such a file will be created automatically. If append = TRUE, has to be a valid path to a file.

base

start of the file name.

Value

list of summarized PTM and Protein results. These results contain the reformatted input to the summarization function, as well as run-level summarization results.

Examples

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head(raw.input$PTM)
head(raw.input$PROTEIN)

quant.lf.msstatsptm <- dataSummarizationPTM(raw.input, verbose = FALSE)
head(quant.lf.msstatsptm$PTM$ProteinLevelData)

devonjkohler/MSstatsPTM documentation built on Dec. 19, 2021, 11:01 p.m.