fold.calc: Analyze qPCR data in R

Description Usage Arguments Value Author(s)

Description

It takes the your date and by computing accordingly to Pfaffl method it return the fold changes

Usage

1
fold.calc(samples, genes, Cts, standev = standev, sd.rm = TRUE, ref, data)

Arguments

samples

A column containing your samples name

genes

A column with your reference and control genes

Cts

Your Ct values

standev

The maximum standard deviation among your technical replicates

sd.rm

A logical parameter to decide whether the outlayer sample will be removed

ref

The reference samples

data

the name of your data frame

Value

Return a five-column data frame

Author(s)

Elves H Duarte


ehelegam/elvesR documentation built on May 16, 2019, 12:15 a.m.