calibrate_sec: Gel filtration column calibration

In gbedwell/calibrateR: Streamline Standard Laboratory Calculations

Gel filtration column calibration

Description

Calculates the calibration curve for a size exclusion column using standards of known mass. Standard mass values should be entered in kDa. Hydrodynamic radius values are calculated from the mass values in nm units. The hydrodynamic radius - molar mass relationship is defined in https://doi.org/10.1046/j.0014-2956.2001.02649.x

Usage

calibrate_sec(
  vols,
  masses,
  parameter = c("mw", "rh"),
  void = NULL,
  cv = NULL,
  normalized = FALSE
)

Arguments

vols	The elution volumes of the standards.
masses	The known masses of each of the standards.
parameter	One of "mw" or "rh". Defines whether the column is being calibrated for molecular weight or hydrodynamic radius.
void	The void volume of the column.
cv	The column volume.
normalized	Boolean. Whether or not the input elution volumes should be normalized relative to the given void and column volumes.

Examples

calibrate_sec( masses, void, cv, vols )

gbedwell/calibrateR documentation built on April 25, 2024, 8:35 p.m.