gibson: Calculate Gibson assembly reaction volumes

In gbedwell/calibrateR: Streamline Standard Laboratory Calculations

Calculate Gibson assembly reaction volumes

Description

Calculate Gibson assembly reaction volumes

Usage

gibson(
  insert.conc,
  insert.len,
  vec.conc,
  vec.len,
  n.frags,
  molar.ratio = 3,
  vec.mass = 50,
  final.vol = 5,
  ids = NULL
)

Arguments

insert.conc	Vector of insert concentrations.
insert.len	Vector of insert lengths in bp.
vec.conc	Vector concentration. Must be a single value.
vec.len	Vector length. Must be a single value.
n.frags	The number of fragments in each reaction.
molar.ratio	Molar ratio of insert:vector
vec.mass	Mass of vector
final.vol	Final volume of the reaction before addition of 2x master mix.
ids	Reaction identifiers. Can be NULL.

Examples

gibson()

gbedwell/calibrateR documentation built on April 25, 2024, 8:35 p.m.