knitr::opts_chunk$set(echo = TRUE)
This document describes the standard method to clean glass and plastic containers prior to collecting most samples or conducting chemical analyses. The basic steps include washing with alkaline soap (Liqui-Nox), acid washing, and, in the case of lab and some field glassware, ashing. Also included in this protocol is making and disposing of a hydrochloric acid (HCl) bath. Based on IsoLab method by A. Schauer.
You will be using a strong alkaline soap (Liqui-Nox) and 12 M hydrochloric acid (HCl), which is a very strong acid. Familiarize yourself with the MSDSs for Liqui-Nox and hydrochloric acid. You must wear closed toed shoes, pants and a lab coat. Safety glasses, a face shield, and long black rubber gloves are required when working with 12 M HCl. Purple nitrile gloves are sufficient protection when working with 1 N acid, although the longer gloves are useful. Material coming out of the muffle furnace can be very hot and there is a chance of shattering if items experience a dramatic change in temperature. Wear eye protection and use metal tongs with heat resistant gloves as necessary.
There are four types of water available on the 2nd floor of FSH. They are:
The default concentration for the acid bath is 1 N (equivalent to 1 M), which is approximately 8% 12 M HCl by volume. Remember to always add acid to water SLOWLY.
Glassware to be used in analysis of organics (i.e., fatty acids or amino acids), as well as some field sampling containers, must also be ashed in the muffle furnace. Brief instructions are given here -- see Muffle Furnace Protocol for detailed instructions. Never muffle volumetric glassware including volumetric flasks, burettes, and pipettes.
Should the acid bath become discolored or contaminated in any way, dispose by first neutralizing then sending down the drain. If contaminated with hazardous waste, follow the Hazardous Waste protocol.
x <- matrix(c("File rendered on:", format(Sys.time(), "%d %b %Y"), "R version:", as.character(getRversion()), "HEEL package version:", as.character(packageVersion("HEEL"))), ncol=2, byrow=T) print(knitr::kable(x, row.names = F))
Add the following code to your website.
For more information on customizing the embed code, read Embedding Snippets.