NormqPCR-package: Functions for normalisation of RT-qPCR data.

In jimrperkins/NormqPCR: Functions for normalisation of RT-qPCR data

Description

Functions for normalisation of real-time quantitative PCR data.

Details

Package:	NormqPCR
Type:	Package
Version:	1.7.1
Date:	2014-08-13
Depends:	R(>= 2.14.0), stats, RColorBrewer, Biobase, methods, ReadqPCR, qpcR
Imports:	ReadqPCR
biocViews:	MicrotitrePlateAssay, GeneExpression, qPCR
License:	LGPL-3
LazyLoad:	yes
LazyData:	yes

require(NormqPCR)

Author(s)

Matthias Kohl, James Perkins, Nor Izayu Abdul Rahman

Maintainer: James Perkins [e-mail]

References

Perkins, JR, Dawes, JM, McMahon, SB, Bennett, DL, Orengo, C, Kohl, M (2012). ReadqPCR and NormqPCR: R packages for the reading, quality checking and normalisation of RT-qPCR quantification cycle (Cq) data. BMC Genomics, 13, 1:296.

Jo Vandesompele, Katleen De Preter, Filip Pattyn et al. (2002). Accurate normalization of real-time quantitative RT-PCR data by geometric averaging of multiple internal control genes. Genome Biology 2002. 3(7):research0034.1-0034.11. http://genomebiology.com/2002/3/7/research/0034/

Claus Lindbjerg Andersen, Jens Ledet Jensen and Torben Falck Orntoft (2004). Normalization of Real-Time Quantitative Reverse Transcription-PCR Data: A Model-Based Variance Estimation Approach to Identify Genes Suited for Normalization, Applied to Bladder and Colon Cancer Data Sets. CANCER RESEARCH 64, 5245-5250, August 1, 2004. http://cancerres.aacrjournals.org/cgi/content/full/64/15/5245

Kenneth Livak, Thomase Schmittgen (2001). Analysis of Relative Gene Expression Data Using Real-Time Quantitative PCR and the 2^ddCt Method. Methods 25, 402-408, 2001 http://www.ncbi.nlm.nih.gov/pubmed/11846609

Examples

## some examples are given in the vignette
## Not run: 
library(NormqPCR)
vignette("NormqPCR")

## End(Not run)

jimrperkins/NormqPCR documentation built on March 6, 2020, 1:06 a.m.