Description Usage Arguments Details Value
Analyse qpcr data
1 2 3 4 5 6 | analyse_qpcr(
qpcr,
reference_genes,
levels = NULL,
aggregation = c("geomean", "mean", "median")
)
|
qpcr |
An instance of the qpcr class. Expression data and sample annotations must have been loaded already. |
reference_genes |
A vector listing the genes one wishes to use as housekeeping genes or reference genes. |
levels |
A list with the set levels for each factor in the sample annotation data. Defaults to NULL. |
aggregation |
Defines how CT values of technical replicates and reference genes should be aggregated; either by their arithmetic ('mean') or geometric mean ('geomean') or their median ('median'). |
analyse_qpcr
allows one to perform the core analysis of qpcr data.
An updated instance of the qpcr class with analysed expression data.
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