analyse_qpcr: analyse_qpcr

Description Usage Arguments Details Value

View source: R/functions.R

Description

Analyse qpcr data

Usage

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analyse_qpcr(
  qpcr,
  reference_genes,
  levels = NULL,
  aggregation = c("geomean", "mean", "median")
)

Arguments

qpcr

An instance of the qpcr class. Expression data and sample annotations must have been loaded already.

reference_genes

A vector listing the genes one wishes to use as housekeeping genes or reference genes.

levels

A list with the set levels for each factor in the sample annotation data. Defaults to NULL.

aggregation

Defines how CT values of technical replicates and reference genes should be aggregated; either by their arithmetic ('mean') or geometric mean ('geomean') or their median ('median').

Details

analyse_qpcr allows one to perform the core analysis of qpcr data.

Value

An updated instance of the qpcr class with analysed expression data.


jonathandesmedt92/RtqpcR documentation built on April 26, 2020, 8:25 a.m.