read_fsa: FSAPlot: read_fsa

In joshuakrobertson/FSAPlot: FSAPlot: a tool for visualising microsatellite amplicons

Description

A function to read in .fsa files from an ABI analyser and produce a data.frame with relative fluoresences according to channel and basepair length.

Usage

read_fsa(File, Lad_Chan = 4, Sig_Chan = 1:3, Range = c(100:500))

Arguments

File	A path to a .fsa file to be opened.
Lad_Chan	The loading channel in which the ladder may be found. Default is 4.
Sig_Chan	Loading channels wherein sample reads lie. Default is 1 to 3.
Range	Basepair range wherein peaks will be extracted. Default is 100 to 500.

Details

The default ladder for fragment length calibration is GS500.

Examples

source = "/home/fish332_microsat.fsa"
Peak_Dat = read_fsa(File = source, Lad_Chan = 4, Range = c(50:80))

joshuakrobertson/FSAPlot documentation built on June 27, 2020, 1:18 a.m.