Main function for calculating colocalization parameters
1 2 3 4 5 6 7 8 9 10 11 12 13 14 15 16 17 18 19 20 21 22 23 24 25 26 27 28 29 30 31 32 33 34 35 36 37 38 39 40 41 42 43 | coloc.Sgl(
MyImCl,
Plate,
Time,
Well,
Site,
Blue = 1,
Green = 2,
Red = 3,
auto1 = T,
auto2 = T,
auto3 = T,
Cyto = "Compt 2",
Nuc.rm = T,
Seg.method = "Fast",
TopSize1 = 29,
TopSize2 = 29,
TopSize3 = 29,
w1OFF = 0.1,
w2OFF = 0.15,
w3OFF = 0.1,
Nuc.denoising = T,
NucWindow = 50,
RO.size = 25,
SegCyto.met = "Automated",
CytoWindow = 50,
CytoOFF = 0.1,
Rm1 = c(0, 0.3),
Rm2 = c(0, 0.1),
Rm3 = c(0, 0.1),
adj = 1,
adj.step1 = 2,
adj.step2 = 2,
adj.step3 = 2,
getCell = F,
add.features = F,
writeSeg = T,
writePDF = F,
TEST = F,
FullIm = F,
getRange = c(F, F, F),
path = "..."
)
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