ExtractProjection: ExtractProjection

Description Usage Arguments Details Value Examples

View source: R/ExtractProjection.R

Description

This function takes a completed GSEPD object with sample data, and a set of gene identifiers and produces the projection of sample expression in the sub-space.

Usage

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ExtractProjection(GSEPD, txids, DRAWING=FALSE, GN=c(1,2), PRINTING=FALSE, plotTitle="")

Arguments

GSEPD

The GSEPD parameter object. Must be post-Process.

txids

The transcript IDs, generally REFSEQ identifiers corresponding to rows of the counts table for this a projection is desired. In normal usage these are based on a GO Term.

DRAWING

Boolean flag to draw a plot of the projection.

GN

The gene numbers: which items of the 'txids' list are to be drawn. Only the first two are used. If Drawing=FALSE, this parameter is irrelevant.

PRINTING

Boolean flag to print some debug information.

plotTitle

A name for this set of genes, serves as the plot's main title.

Details

Primary gene set projection tool. This function calculates the vector projection and axis in a N-dimensional space of gene expression for a set of samples. When DRAWING=TRUE you will get some diagrams of the expression normalized counts.

Value

Returns a list object with four values for each sample.

alpha

Distance along the axis from group1 to group2, generally 0-1, as in percent. Samples within group 1 should average zero, and samples in group 2 should average one.

beta

Distance from the samples to the axis. This is a measure of goodness of fit, when the value is zero it means the sample is a linear interpolation between the comparison groups. When the value is high, the sample is not along the n-dimensional axis.

gamma1

Distance from the samples to the center of group1

gamma2

Distance from the samples to the center of group2

Validity.Score

A score, 0% through 100%, of the segregation validity for this gene set among the two sample test groups.

Validity.P

The validity score's associated p-value, empirically calculated chance of a random sample assignment creating such a strong score.

Examples

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  data("IlluminaBodymap")
  data("IlluminaBodymapMeta")
  set.seed(1000) #fixed randomness
  isoform_ids <- Name_to_RefSeq(c("HIF1A","EGFR","MYH7","CD33","BRCA2"))
  rows_of_interest <- unique( c( isoform_ids ,
                                 sample(rownames(IlluminaBodymap),
                                        size=2000,replace=FALSE)))
  G <- GSEPD_INIT(Output_Folder="OUT",
                finalCounts=round(IlluminaBodymap[rows_of_interest , ]),
                sampleMeta=IlluminaBodymapMeta,
                COLORS=c("green","black","red"))
                
  G <- GSEPD_ChangeConditions( G, c("A","B")) #set testing groups first!    
  G <- GSEPD_Process( G ) #have to have processed results to plot them
   
   # looking at genes 2 and 3 will show us a view in dimensions "EGFR" and "MYH7"
   # and an axis through five dimensional space.
  ExtractProjection(GSEPD=G, txids=isoform_ids, 
    DRAWING=TRUE, PRINTING=TRUE, GN=c(2,3))
  

kstammits/rgsepd documentation built on July 4, 2020, 2:08 p.m.