gathmann.bt | R Documentation |
Impact of Bt corn on non-target species
A data frame with 16 observations on the following 3 variables.
gen
genotype/maize, Bt
ISO
thysan
thysan abundance
aranei
aranei abundance
The experiment involved comparing a Bt maize and a near-isogenic control variety.
Species abundances were measured for Thysanoptera (thrips) and Araneida (spiders) in 8 different plots.
Each response is probably a mean across repeated measurements.
Used with permission of Achim Gathmann.
L. A. Hothorn, 2005. Evaluation of Bt-Maize Field Trials by a Proof of Safety. https://www.seedtest.org/upload/cms/user/presentation7Hothorn.pdf
## Not run:
library(agridat)
data(gathmann.bt)
dat <- gathmann.bt
# EDA suggests Bt vs ISO is significant for thysan, not for aranei
libs(lattice)
libs(reshape2)
d2 <- melt(dat, id.var='gen')
bwplot(value ~ gen|variable, d2,
main="gathmann.bt", ylab="Insect abundance",
panel=function(x,y,...){
panel.xyplot(jitter(as.numeric(x)),y,...)
panel.bwplot(x,y,...)
},
scales=list(relation="free"))
if(0){
# ----- Parametric CI. Thysan significant, aranei not significant.
libs(equivalence)
th0 <- with(dat, tost(thysan[1:8], thysan[9:16], alpha=.05, paired=FALSE))
lapply(th0[c("estimate","tost.interval")], round, 2)
# 14.28-8.72=5.56, (2.51, 8.59) # match Gathmann p. 11
ar0 <- with(dat, tost(aranei[1:8], aranei[9:16], alpha=.05, epsilon=.4))
lapply(ar0[c("estimate","tost.interval")], round, 2)
# .57-.47=.10, (-0.19, 0.40) # match Gathmann p. 11
# ----- Non-parametric exact CI. Same result.
libs(coin)
th1 <- wilcox_test(thysan ~ gen, data=dat, conf.int=TRUE, conf.level=0.90)
lapply(confint(th1), round, 2)
# 6.36, (2.8, 9.2) # Match Gathmann p. 11
ar1 <- wilcox_test(aranei ~ gen, data=dat, conf.int=TRUE, conf.level=0.90)
lapply(confint(ar1), round, 2)
# .05 (-.2, .4)
# ----- Log-transformed exact CI. Same result.
th2 <- wilcox_test(log(thysan) ~ gen, data=dat, alternative=c("two.sided"),
conf.int=TRUE, conf.level=0.9)
lapply(confint(th2), function(x) round(exp(x),2))
# 1.66, (1.38, 2.31) # Match Gathmann p 11
# ----- Log-transform doesn't work on aranei, but asinh(x/2) does
ar2 <- wilcox_test(asinh(aranei/2) ~ gen, data=dat,
alternative=c("two.sided"),
conf.int=TRUE, conf.level=0.9)
lapply(confint(ar2), function(x) round(sinh(x)*2,1))
}
## End(Not run)
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