plotRangesLinkedToData | R Documentation |
Plot GRanges object structure and linked to a even spaced paralell coordinates plot which represting the data in elementeMetadata.
## S4 method for signature 'RangedSummarizedExperiment'
plotRangesLinkedToData(data, ...,
stat.y = seq_len(ncol(data)), stat.ylab = names(assays(data)[stat.assay]),
stat.assay = 1L)
## S4 method for signature 'GenomicRanges_OR_GRangesList'
plotRangesLinkedToData(data, ...,
stat.y = seq_len(ncol(mcols(data))),
stat.ylab, sig, sig.col = c("black", "red"),
stat.coord.trans = coord_trans(),
annotation = list(), width.ratio = 0.8,
theme.stat = theme_gray(), theme.align = theme_gray(),
linetype = 3, heights)
data |
GRanges object with a DataFrame as elementMetadata. |
... |
Parameters passed to control lines in top plot. |
stat.y |
integer (variable position starting in DataFrame of data, start from 1) or strings (variable names) which indicate the column names. |
stat.ylab |
y label for stat track(the top track). |
stat.assay |
default 1L, element of assays. |
sig |
a character of element meta data column of logical value, indicates which row is signficant. and will be shown in link lines and rectangle. |
sig.col |
colors for significant, valid when you specify "sig" argument, the
first color indicates |
stat.coord.trans |
transformation used for top plot. |
annotation |
A list of ggplot object. |
width.ratio |
Control the segment length of statistic layer. |
theme.stat |
top plot theme. |
theme.align |
alignment themes. |
linetype |
linetype |
heights |
Heights of each track. |
Inspired by some graphics produced in some other packages, for example
in package DEXseq, the author provides graphics with gene
models and linked to an even spaced statistics summary. This is useful
because we always plot everything along the genomic coordinates, but
genomic features like exons are not evenly distributed, so we could
actually treat the statistics associated with exons like categorical
data, and show them as "Paralell Coordinates Plots". This is one
special layout which represent the data in a nice manner and also keep
the genomic structure information. With abliity of tracks
,
it's possible to generate such type of a graphic along with other
annotations.
The data we want is a normal GRanges
object, and make sure
the intervals are not overlaped with each other(currently), and you
may have multiple columns which store the statistics for multiple
samples, then we produce the graphic we introduced above and users
could pass other annotation track in the function which will be shown
below the main linked track.
The reason you need to pass annotation into the function instead of
binding them by tracks
later is because binding manually
with annotation tracks is tricky and this function doesn't return a
ggplot object.
return a frame grob; side-effect (plotting) if plot=T.
Tengfei Yin
library(TxDb.Hsapiens.UCSC.hg19.knownGene)
library(ggbio)
data(genesymbol, package = "biovizBase")
txdb <- TxDb.Hsapiens.UCSC.hg19.knownGene
model <- exonsBy(txdb, by = "tx")
model17 <- subsetByOverlaps(model, genesymbol["RBM17"])
exons <- exons(txdb)
exon17 <- subsetByOverlaps(exons, genesymbol["RBM17"])
## reduce to make sure there is no overlap
## just for example
exon.new <- reduce(exon17)
## suppose
values(exon.new)$sample1 <- rnorm(length(exon.new), 10, 3)
values(exon.new)$sample2 <- rnorm(length(exon.new), 10, 10)
values(exon.new)$score <- rnorm(length(exon.new))
values(exon.new)$significant <- sample(c(TRUE,FALSE), size = length(exon.new),replace = TRUE)
plotRangesLinkedToData(exon.new, stat.y = c("sample1", "sample2"))
plotRangesLinkedToData(exon.new, stat.y = 1:2)
plotRangesLinkedToData(exon.new, stat.y = 1:2, size = 3, linetype = 4)
plotRangesLinkedToData(exon.new, stat.y = 1:2, size = 3, linetype = 4,
sig = "significant")
plotRangesLinkedToData(exon.new, stat.y = 1:2, size = 3, linetype = 4,
sig = "significant", sig.col = c("gray90","red"))
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