library(shiny)
library(plotly)
library(data.table)
library(readr)
library(ggplot2)
library(tidyr)
library(dplyr)
library(GGally)
library(edgeR)
server <- function(input, output, session) {
# coty <- read_delim("../../R/SISBID-2016-master/data/GSE61857_Cotyledon_normalized.txt.gz", delim="\t", col_types="cddddddddd", col_names=c("ID", "C_S1_R1", "C_S1_R2", "C_S1_R3", "C_S2_R1", "C_S2_R2", "C_S2_R3", "C_S3_R1", "C_S3_R2", "C_S3_R3"), skip=1)
# coty <- as.data.frame(coty)
#
# d <- DGEList(counts = coty[,2:7],
# group = c(rep("S1", 3), rep("S2", 3)),
# genes = coty[,1])
# d <- calcNormFactors(d)
# d <- estimateCommonDisp(d)
# d <- estimateTagwiseDisp(d)
# d <- estimateTrendedDisp(d)
# de <- exactTest(d, pair=c("S1", "S2"), dispersion = "tagwise")
# sig.tab <- de$table
# sig.tab$genes <- coty$ID
# sig.tab <- sig.tab %>% filter(PValue < 0.01)
#
# sig.tab <- merge(sig.tab, coty[,1:7], by.x="genes", by.y="ID")
#
# sig.tab <- mutate(sig.tab, xmin = pmin(C_S1_R1, C_S1_R2, C_S1_R3), xmax = pmax(C_S1_R1, C_S1_R2, C_S1_R3), ymin = pmin(C_S2_R1, C_S2_R2, C_S2_R3), ymax = pmax(C_S2_R1, C_S2_R2, C_S2_R3))
load("sigTab.rda")
output$plot <- renderPlotly({
p <- ggplot(sig.tab, aes(xmin = xmin, xmax = xmax, ymin = ymin, ymax = ymax)) + geom_rect(fill = 'purple', color = 'black', size = 0.3, alpha = 0.1)
ggplotly(p)
})
# hover, click --> Return only cruveNumber: int 0
# relayout --> Return four corners of literal selection
# selected --> ?
d <- reactive(event_data("plotly_relayout"))
output$click <- renderPrint({
if (is.null(d())){
"Click on a state to view event data"
}
else{
str(d())
# d()$pointNumber
# dat[(d()$pointNumber+1),]
# dat[(d()$pointNumber+1),]$ID
# str(dat[(d()$pointNumber+1),]$ID)
}
})
# Convert DF from scatterplot to PCP
# datt <- data.frame(t(dat))
# names(datt) <- as.matrix(datt[1, ])
# datt <- datt[-1, ]
# datt[] <- lapply(datt, function(x) type.convert(as.character(x)))
# setDT(datt, keep.rownames = TRUE)[]
# colnames(datt)[1] <- "x"
# dat_long <- melt(datt, id.vars ="x" )
# output$plot2 <- renderPlotly({
# plot_ly(dat_long[dat_long$variable %in% dat[(d()$pointNumber+1),]$ID,], x= ~x, y= ~value, type = 'scatter', mode = 'lines+markers', color = ~variable) %>% layout(dragmode="box", showlegend = FALSE)
# })
}
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