1 | BED12toGRangesList(filepath)
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filepath |
1 2 3 4 5 6 7 8 9 10 11 12 13 14 15 16 17 18 19 20 21 22 23 24 25 26 27 28 29 30 31 32 33 | ##---- Should be DIRECTLY executable !! ----
##-- ==> Define data, use random,
##-- or do help(data=index) for the standard data sets.
## The function is currently defined as
function (filepath)
{
print("Converting BED12 to GRangesList")
print("It may take a few minutes")
a = read.table(filepath, sep = "\t", header = TRUE, stringsAsFactors = FALSE)
a = a[, 1:12]
no_tx = length(a[, 1])
tx_id = 1:no_tx
tx_name = paste("line1", 1:no_tx, sep = "")
tx_chrom = a[, 1]
tx_strand = a[, 6]
tx_start = a[, 2] + 1
tx_end = a[, 3]
transcripts = data.frame(tx_id, tx_name, tx_chrom, tx_strand,
tx_start, tx_end)
head(transcripts)
tx_name = tx_name
gene_id = as.character(a[, 4])
gene_id[is.na(gene_id)] = "NA"
gene = data.frame(tx_name, gene_id)
splicing <- lapply(1:no_tx, .spliceSingleTrans, a = a, tx_start = tx_start)
splicing <- .combineListOfSplicing(splicing)
peaks = suppressWarnings(makeTxDb(transcripts = transcripts,
splicings = splicing, genes = gene))
tx <- exonsBy(peaks, "tx", use.names = TRUE)
mcols(tx) <- a
return(tx)
}
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