gate_facs_yeast_HTS_data: Interrogates the FSC/SSC density plot and extract ncell from...

In magrichard/facsticor: Treatment and analysis of FACS data during time course experiments

Description

This function works of FL1H, FSC and SSC data acquired on 96-well plates

Usage

gate_facs_yeast_HTS_data(data, frac_cells = 0.4, graphics = TRUE,
  pre_gating_rm_rate = 0.05, verbose = TRUE, hard_lim = NULL)

Arguments

data	A list containing a field called alldat, generated by the read_data.R function
pre_gating_rm_rate	Pre-gating fraction of cells that will be remove on x and y axis. This allows to limit computations and filtering to the area of interest. Yeast cells being small, default value is 0.05.
frac_cell	A fraction of cells to keep after gating (approximately). This number defines the size of the gate (few cells: narrow gate).
graphic	If TRUE (default), graphics are produced in pdf files to visualize gating. The name of these files will be in the format platewell.pdf.
graphicsdir	A character string giving the path to the directory where the graphics files should be written

Value

A list of data.frames containing the data of GATED cells. data$gdat in a list of gated data, and data$anno is the summary of the informations, it now contains the summary of gated data.

magrichard/facsticor documentation built on May 17, 2019, 8:16 a.m.