inst/MFIaggr.gui/readme.md

About

MFIaggr is used for a fast multiple comparison of the cycle dependent variance of the fluorescence. It is a web-based implementation of function from package chipPCR.

Authors: Stefan Roediger, Michal Burdukiewicz.

Readme

  1. Choose file - a .csv file with a fluorescence data and cycle number.
  2. Header - must be checked if input table has header.
  3. Type of .csv file - choose type of .csv file (depending on decimal separator).
  4. Column containing the cycle data - number of column containing the cycle number.
  5. Relative standard deviation - show relative standard deviation in percent.
  6. Median and MAD - must be checked if median and MAD should be used instead of mean and standard deviation.
  7. Lower and upper border of the region of interest - two cycle numbers defining upper and lower limit of the region of interest (ROI) used for the density and quantile plot.


michbur/chipPCR documentation built on Feb. 28, 2021, 5:57 p.m.