sink_bmg: Import cell sinking data from BMG Labtech plate...

Description Usage Arguments Value Examples

Description

Transforms sinking data from plate format into tidy format. Works on .txt and .csv output from FLUOStar Omega and CLARIOStar plate readers.

Usage

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sink_bmg(data_folder, catalog = NA, time_units = "sec", RFU_cutoff = 10, skip = 1,
export_output = FALSE, save_as = "Sink_Database.csv")

Arguments

data_folder

folder containing the data files to be read, in quotations. Should be in the working directory.

catalog

optional dataframe containing experimental metadata that can be merged with the imported data files. Should include a "Plate" column containing the filename (including file extension if .csv), and "Well" in format A1, A2, etc.

time_units

units of time in which measurements were taken (either "sec", "min", or "hr").

RFU_cutoff

threshold for RFU values to be kept.

skip

lines to be skipped when reading raw data files; should be sufficient to skip headers/blank lines.

export_output

whether the resulting dataframe should be exported to .csv.

save_as

desired filename, if results are to be exported.

Value

A single dataframe containing tidied data from all files in data_folder, as well as experimental metadata if catalog is supplied. Creates the columns "Well", "Elapsed.Time.m", "RFU", and "Plate" (filename without extension).

Examples

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sink_bmg("Sinking BMG", time_units = "min", RFU_cutoff = 7)

mlrioux/sinkworx documentation built on June 2, 2020, 10:28 p.m.