analyzeassay.standard: Quick Analysis

In mohrbp/AnalyzeAssay: What the Package Does (One Line, Title Case)

Quick Analysis

Description

This method uses the import.assaydat, dereplicate, subtract.background, normalize.bysignal and report.assaymax functions to quickly process a standard dataset

Usage

analyzeassay.standard(
  PathtoDatInfile,
  PathtoIds,
  GroupBy = c("DNA", "Protein", "Minutes"),
  BackgroundSignalType = Protein,
  NameofBackgroundSignal = "B",
  NormalizationSignalType = Protein,
  NameofNormalizationSignal = "C",
  SortGroup = "Protein",
  Writecsv = FALSE,
  Plot = TRUE,
  SavePlot = FALSE
)

Arguments

PathtoDatInfile	A list of path names to the assay data to be imported.
PathtoIds	A path to the file of identifiers for the assay data.
GroupBy	The names of the variables over which the assay data is separated including minutes. Defaults to DNA, Protein and Minutes. Should not include "Replicate"
BackgroundSignalType	The variable type of the signal to subtract from all other signals
NameofBackgroundSignal	The quoted name of the signal to subtract from all other signals
NormalizationSignalType	The variable type of the signal to divide all other signals
NameofNormalizationSignal	The quoted name of the signal to divide all other signals
SortGroup	The descriptive variable contained in the identifier file used to sort. Quoted
Writecsv	A logical of whether to output a .csv in the current directory titled YYYYMMDD

Value

A report of the max normalized and background subtracted signal for the chosen group.

mohrbp/AnalyzeAssay documentation built on Oct. 2, 2022, 5:54 p.m.