Elastase: Peptide identification results of HeLa cells digested with...

In ohgane/DigestionSpecificity: Analyze Cleavage Site Specificity

Description

Identification results from a bottom-up proteomics experiment. HeLa cell lysate was digested with Elastase using TFE digestion protocol on an AmiconUltra 10K, and the digest was analyzed on a Thermo LTQ-Orbitrap XL in data-dependent acquisition mode (orbitrap detector for MS1 at 60000 resolution, and ion trap for MS2). The obtained data was searched against a subset of human protein database (from uniprot, ~1500 sequences) by using SearchGUI (X!Tandem and OMSSA, unspecific search, precursor tolerance 4 ppm, and fragment ion tolerance 0.4 Da). The identification results were processed with PeptideShaker (FDR 1

Usage

data(Elastase)

Format

An object of class data.frame, with its rows corresponding to each peptide identifications. Its columns include Sequence, AAs.After etc.

See Also

LysCTryp

Examples

data(Elastase)
head(Elastase)

ohgane/DigestionSpecificity documentation built on May 24, 2019, 11:55 a.m.