fluorescence_to_zscore_matrix: Transform fluorescence matrix to z-scores

Description Usage Arguments Details Value Examples

View source: R/zscore_matrix.R

Description

Converts a data frame of fluorescence values into PBM condition-wise z-scores using the background fluorescence values.

Usage

1
fluorescence_to_zscore_matrix(annotated_fluorescence_matrix)

Arguments

annotated_fluorescence_matrix

A data frame of fluorescence values and annotations for each probe, such as output by annotate_fluorescence_matrix().

Details

This function normalizes raw fluorescence values against the fluorescence values of the background probes. It first log-transforms all the fluorescence values for a given PBM condition, then calculates a z-score like statistic using the mean and standard deviation of the background probes.

Value

A data frame of PBM condition-wise fluorescence value z-scores.

Examples

1
zscore_matrix <- fluorescence_to_zscore_matrix(annotated_fluorescence_matrix)

pamelaglopez/hTF_array documentation built on Feb. 23, 2022, 12:05 a.m.