Description Usage Arguments Details Value Examples
View source: R/zscore_matrix.R
Converts a data frame of fluorescence values into PBM condition-wise z-scores using the background fluorescence values.
1 | fluorescence_to_zscore_matrix(annotated_fluorescence_matrix)
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annotated_fluorescence_matrix |
A data frame of fluorescence values and annotations for each probe, such as output by annotate_fluorescence_matrix(). |
This function normalizes raw fluorescence values against the fluorescence values of the background probes. It first log-transforms all the fluorescence values for a given PBM condition, then calculates a z-score like statistic using the mean and standard deviation of the background probes.
A data frame of PBM condition-wise fluorescence value z-scores.
1 | zscore_matrix <- fluorescence_to_zscore_matrix(annotated_fluorescence_matrix)
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