Description Usage Arguments Value Examples
Rank Based Differential Expression Analysis This function identifies differentially expressed genes from a raw count table
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countData |
A raw count table (dataframe or matrix) with samples as columns and genes as rows. |
colData |
A dataframe with samples as rows and columns factor variables indicating experimental groups. |
testVariable |
Character indicating the column name in colData that reresents the variable of interest for differential expression analysis. |
batch_family_variable |
This paramater is used in N=1 cases only. Character that specifies a column name in colData that indicates other samples sequenced in the same batch as the sample of interest or related family members. It can be used as a downstream filter when comparing one sample to a large reference set. Defaults to NULL. |
randomSeed |
A numeric indicating a random seed for reproducible analysis. Defaults to 1990 |
minP |
A numeric between 0 and one indicating the minimum possible P-value computed via random sampling from the countTable. The larger this number is the faster the compute time will be. Defaults to 0.000005. |
numCores |
A numeric indicating the number of cores used for computing. Most laptops can readily handle up to 4. Defaults to 4. |
Returns a data frame containing differential expression results. The first column, "variance_rank," indicates the percentile rank of a gene's rank variance. Low numbers indicate a gene exhibits very low variance across samples. The second column "test_statistic" is the RBDA test statistic. The third column "p_value" indicates the probability of observing the test statistic by chance alone. A fourth column, "min_batch_fam_p_value," will only result if the batch_family_variable parameter is specified and will provide the minimum p-value computed for any samples indicated by this factor variable in colData.
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