inst/guides/Guide.to.signature.attribution.md

In steverozen/mSigAct.server: Shiny Server for Analyzing Mutational Signature Activities

Table of contents

• Input
• Pre-loaded example spectra
• Use which signatures for attribution?
• What reference genome to use?
• Upload spectra
• Show the spectra or start the attribution analysis
• Choose cancer type
• Add or delete signatures from the analysis?
• Look at the mutational signatures under consideration
• Start attribution analysis
• Examine the analysis results
• Attribution for other mutation types

Guide to signature attribution in the mSigAct web server

The tab “Signature attribution” provides analytical tools to estimate which mutational signatures have contributed to the mutational spectrum of a single tumor or tissue sample.

The input area for uploading spectra catalogs

You can also analyze spectra catalogs generated from VCFs in the mSigAct web server.

Pre-loaded example spectra and example spectra catalogs to download then update

Important: genome or exome data?

What reference genome to use?

Upload the spectra from a .csv file

You can show the spectra or start the attribution analysis

mSigAct needs to know the cancer type to decide what signatures to consider

Add or delete signatures from the analysis?

Look at the mutational signatures under consideration

Start the analysis

Examine the analysis results

Attribution for other mutation types is similar

Here is an analysis of insertion and deletion signature attribution.

steverozen/mSigAct.server documentation built on July 9, 2023, 4:52 a.m.