kaksAA: Compute kaks for individual amino mutation

In superkits/CorMut: Detect the correlated mutations based on selection pressure

Description

Compute kaks for individual amino mutation.

Usage

kaksAA(seq_formated)

Arguments

seq_formated

Formated alignment sequence. i.e. the result after the treatment of DataFormatCorMut.

Details

Ka/Ks ratio was used as an indicator of selective pressure acting on a protein-coding gene. A Ka/Ks ratio of 1 indicates neutral selection, i.e., the observed ratio of non-synonymous mutations versus synonymous mutations exactly matches the ratio expected under a random mutation model. Thus, amino acid changes are neither being selected for nor against. A Ka/Ks value of <1 indicates negative selection pressure. That is to say most amino acid changes are deleterious and are selected against, producing an imbalance in the observed mutations that favors synonymous mutations. In the condition of Ka/Ks>1 , it indicates that amino acid changes are favored, i.e., they increase the organism's fitness. This unusual condition may reflect a change in the function of a gene or a change in environmental conditions that forces the organism to adapt. For example, highly variable viruses mutations which confer resistance to new antiviral drugs might be expected to undergo positive selection in a patient population treated with these drugs, such as HIV and HCV.

Value

A kaksAA instance will be returned.

Note

The reference sequence should be included in in the alignment as the first sequence.Before using kaksCodon, the alignment should be treated with DataFormatCorMut.

Author(s)

Zhenpeng Li

References

Chen, L., Perlina, A. & Lee, C. J. Positive selection detection in 40,000 human immunodeficiency virus (HIV) type 1 sequences automatically identifies drug resistance and positive fitness mutations in HIV protease and reverse transcriptase. Journal of virology 78, 3722-3732 (2004).

See Also

filterSites,kaksCodon

Examples

examplefile=system.file("extdata","PI_treatment.aln",package="CorMut")
example=seqFormat(examplefile)
result=kaksAA(example)

superkits/CorMut documentation built on May 8, 2019, 11:59 p.m.