read_qtower: Read raw data file as exported from a qTower

In taiawu/dsfworld_package: Visualizes and Analyzes Differential Scanning Fluorimetry Data

Read raw data file as exported from a qTower

Description

Read raw data file (.csv) directly as exported from a qTower 384G qPCR instrument. Creates a "channel_f" column, which contains the channels as a factor, with default levels of FAM, JOE, TAMRA, ROX, Cy5 ,Cy5.5, SyproOrange. Converts from cycle number to Temperature, with a default starting temperature of 25, and 1 C increase between measurements.

Usage

read_qtower(
  file_path,
  start_temp = 25,
  inc_temp = 1,
  channel_levels = c("FAM", "JOE", "TAMRA", "ROX", "Cy5", "Cy5.5", "SyproOrange")
)

Arguments

file_path	A complete file path, pointing to the raw data (.csv) exported from a qTower
start_temp	The temperature of the first read in the data file. Used to convert from cycle number to Temperature
inc_temp	The increment by which the temperature increases with each read. Used to convert from cycle number to Temperature.
channel_levels	A vector used to set the order of the channels measured in the loaded experiment. Defaults to FAM, JOE, TAMRA, ROX, Cy5 ,Cy5.5, SyproOrange. Channels not present in this list will be NA in the output tibble.

Value

A tibble containg all data, but no meta-data, present in the loaded csv file. Includes columns: variable (combines well and channel, to uniquely identify each trace), well, channel, channel_f (channels as a factor, ordered by increasing excitation wavelength), Temperature, value, and value_norm (each raw data trace, rescaled to a 0 to 1 range).

taiawu/dsfworld_package documentation built on June 18, 2024, 5:39 a.m.