negativeControl: Acessor functions for the 'negativeControl' slot in a...
In tingtina/NanoStringDiff: Differential Expression Analysis of NanoString nCounter Data
Description
Usage
Arguments
Details
Value
Author(s)
See Also
Examples
Description
Negative control genes are provided by nCounter Analyzer which can be used to
estimate background noise for each sample.
Usage
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## S4 method for signature 'NanoStringSet'
negativeControl(object)
## S4 replacement method for signature 'NanoStringSet,matrix'
negativeControl(object) <- value
Arguments
object
A NanoStringSet object.
value
A matrix with negative control genes.
Details
Each code set in the nCounter Analyzer includes several negatives
control genes for which no tranCounterript is expected to be present.
We use these spike-in negative control genes to estimate
background noise for each sample.
Value
A matrix contain negative control genes
Author(s)
Hong Wang <hong.wang@uky.edu>
chi wang <chi.wang@uky.edu>
See Also
negativeFactor
Examples
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data(NanoStringData)
## obtain negative control genes
negativeControl(NanoStringData)
## assign a matrix
n=ncol(exprs(NanoStringData))
r=nrow(negativeControl(NanoStringData))
negative=matrix(rpois(r*n,10),ncol=n)
negativeControl(NanoStringData)=negative
tingtina/NanoStringDiff documentation built on May 24, 2019, 10 p.m.
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Description Usage Arguments Details Value Author(s) See Also Examples
Description
Negative control genes are provided by nCounter Analyzer which can be used to estimate background noise for each sample.
Usage
1 2 3 4 | ## S4 method for signature 'NanoStringSet'
negativeControl(object)
## S4 replacement method for signature 'NanoStringSet,matrix'
negativeControl(object) <- value
|
Arguments
object |
A NanoStringSet object. |
value |
A matrix with negative control genes. |
Details
Each code set in the nCounter Analyzer includes several negatives control genes for which no tranCounterript is expected to be present. We use these spike-in negative control genes to estimate background noise for each sample.
Value
A matrix contain negative control genes
Author(s)
Hong Wang <hong.wang@uky.edu> chi wang <chi.wang@uky.edu>
See Also
negativeFactor
Examples
1 2 3 4 5 6 7 8 9 | data(NanoStringData)
## obtain negative control genes
negativeControl(NanoStringData)
## assign a matrix
n=ncol(exprs(NanoStringData))
r=nrow(negativeControl(NanoStringData))
negative=matrix(rpois(r*n,10),ncol=n)
negativeControl(NanoStringData)=negative
|
R Package Documentation
Browse R Packages
We want your feedback!
Note that we can't provide technical support on individual packages. You should contact the package authors for that.
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