knitr::opts_chunk$set(fig.pos = 'H', fig.align = "center", warning = FALSE, message = FALSE)
The r Biocpkg("GBScleanR")
package has been developed to conduct error correction on genotype data obtained via NGS-based genotyping methods such as RAD-seq and GBS [@Miller2007; @Elshire2011]. It is designed to estimate true genotypes along chromosomes from given allele read counts in the VCF file generated by SNP callers like GATK and TASSEL-GBS [@McKenna2010; @Glaubitz2014]. The current implementation supports genotype data of a mapping population derived from two or more diploid parents followed by selfings, sibling crosses, or random crosses. e.g. F$_2$ and 8-way RILs. Our method supposes markers to be biallelic and ordered along chromosomes by mapping reads on a reference genome sequence. To support smooth access to large size genotype data, every input VCF file is first converted to a Genomic Data Structure (GDS) file [@Zheng2012]. The current implementation does not allow non-biallelic markers, and those markers in an input VCF file will be automatically removed from a resultant GDS file. r Biocpkg("GBScleanR")
also provides functions for data visualization, filtering, and loading/writing a VCF file. Furthermore, the data structure of the GDS file created via this package is compatible with those used in the r Biocpkg("SNPRelate")
, r Biocpkg("GWASTools")
and r Biocpkg("GENESIS")
packages those are designed to handle large variant data and conduct downstream analyses including regression analysis [@Zheng2012; @Gogarten2012; @Gogarten2019]. In this document, we first walk through the utility functions implemented in r Biocpkg("GBScleanR")
to introduce a basic usage. Then, the core function of r Biocpkg("GBScleanR")
which estimates true genotypes for error correction will be introduced.
This package internally uses the following packages.
- r CRANpkg("ggplot2")
- r CRANpkg("dplyr")
- r CRANpkg("tidyr")
- r CRANpkg("expm")
- r Biocpkg("gdsfmt")
- r Biocpkg("SeqArray")
\
You can install r Biocpkg("GBScleanR")
from the Bioconductor repository with the following code.
if (!requireNamespace("BiocManager", quietly = TRUE)) install.packages("BiocManager") BiocManager::install("GBScleanR")
\
The code below let you install the package from the github repository. The package released in the github usually get frequent update more than that in Bioconductor due to the release schedule.
if (!requireNamespace("devtools", quietly = TRUE)) install.packages("devtools") devtools::install_github("tomoyukif/GBScleanR", build_vignettes = TRUE)
You may face to the following error message or similar one if you killed the process that was accessing a GDS file.
Stream Read Error, need 12 byte(s) but receive 0
This error message indicates the corruption of the GDS file and you cannot access it anymore.
In this case, please remake a GDS file using the gbsrVCF2GDS()
function.
The main class of the r Biocpkg("GBScleanR")
package is GbsrGenotypData
which inherits the GenotypeData
class in the r Biocpkg("SeqArray")
package. The gbsrGenotypeData
class object has three slots: sample
, marker
, and scheme
. The data
slot holds genotype data as a gds.class
object which is defined in the gdsfmt
package while snpAnnot
and scanAnnot
contain objects storing annotation information of SNPs and samples, which are the SnpAnnotationDataFrame
and ScanAnnotationDataFrame
objects defined in the r Biocpkg("GWASTools")
package. See the vignette of r Biocpkg("GWASTools")
for more detail. r Biocpkg("GBScleanR")
follows the way of r Biocpkg("GWASTools")
in which a unique genotyping instance (genotyped sample) is called "scan".
\
Load the package.
library("GBScleanR")
\
r Biocpkg("GBScleanR")
only supports a VCF file as input. As an example data, we use sample genotype data for a biparental F2 population derived from inbred parents.
vcf_fn <- system.file("extdata", "sample.vcf", package = "GBScleanR") gds_fn <- tempfile("sample", fileext = ".gds")
\
As mentioned above, the GbsrGenotypeData
class requires genotype data in the gds.class
object which enable us quick access to the genotype data without loading the whole data on RAM. At the beginning of the processing, we need to convert data format of our genotype data from VCF to GDS. This conversion can be achieved using gbsrVCF2GDS()
as shown below. A compressed VCF file (.vcf.gz) is also acceptable.
# `force = TRUE` allow the function to over write the GDS file, # even if a GDS file exists at `out_fn`. gbsrVCF2GDS(vcf_fn = vcf_fn, out_fn = gds_fn, force = TRUE, verbose = FALSE)
\
Once we converted the VCF to the GDS, we can create a GbsrGenotypeData
instance for our data.
gds <- loadGDS(gds_fn, verbose = FALSE)
The first time to load a newly produced GDS file will take long time due to data reformatting for quick access.
Getter functions allow you to retrieve basic information of genotype data, e.g. the number of SNPs and samples, chromosome names, physical position of SNPs and alleles.
# Number of samples nsam(gds)
\
# Number of SNPs nmar(gds)
\
# Indices of chromosome ID of all markers head(getChromosome(gds))
\
# Chromosome names of all markers head(getChromosome(gds))
\
# Position (bp) of all markers head(getPosition(gds))
\
# Reference allele of all markers head(getAllele(gds))
\
# SNP IDs head(getMarID(gds))
\
# sample IDs head(getSamID(gds))
\
The function getGenotype()
returns genotype call data in which integer numbers 0, 1, and 2 indicate the number of reference allele.
geno <- getGenotype(gds)
\
The function getRead()
returns read count data as a list with two elements ref
and alt
containing reference read counts and alternative read counts, respectively.
geno <- getRead(gds)
countGenotype()
and countRead()
are class methods of GbsrGenotypeData
and they summarize genotype counts and read counts per marker and per sample.
gds <- countGenotype(gds) gds <- countRead(gds)
\
These summary statistics can be visualized via plotting functions.
With the values obtained via countGenotype()
, we can plot histograms of missing rate , heterozygosity, reference allele frequency as shown below.
# Histgrams of missing rate histGBSR(gds, stats = "missing")
\
# Histgrams of heterozygosity histGBSR(gds, stats = "het")
\
# Histgrams of reference allele frequency histGBSR(gds, stats = "raf")
\
With the values obtained via countRead()
, we can plot histograms of total read depth , allele read depth , reference read frequency as shown below.
# Histgrams of total read depth histGBSR(gds, stats = "dp")
\
# Histgrams of allelic read depth histGBSR(gds, stats = "ad_ref")
\
# Histgrams of allelic read depth histGBSR(gds, stats = "ad_alt")
\
# Histgrams of reference allele frequency histGBSR(gds, stats = "rrf")
\
# Histgrams of mean allelic read depth histGBSR(gds, stats = "mean_ref")
\
# Histgrams of standard deviation of read depth histGBSR(gds, stats = "sd_ref")
\
# Histgrams of standard deviation of read depth histGBSR(gds, stats = "sd_ref")
\
plotGBSR()
and pairsGBSR()
provide other ways to visualize statistics. plotGBSR()
draws a line plot of a specified statistics per marker along each chromosome. pairsGBSR()
give us a two-dimensional scatter plot to visualize relationship between statistics.
plotGBSR(gds, stats = "missing")
\
plotGBSR(gds, stats = "geno")
\
pairsGBSR(gds, stats1 = "missing", stats2 = "dp")
\
The statistics obtained via countGenotype()
, countReat()
, and calcReadStats()
are sotred in the snpAnnot
and scanAnnot
slots. They can be retrieved using getter functions as follows.
# Reference genotype count per marker head(getCountGenoRef(gds, target = "marker")) # Reference genotype count per sample head(getCountGenoRef(gds, target = "sample"))
\
# Heterozygote count per marker head(getCountGenoHet(gds, target = "marker")) # Heterozygote count per sample head(getCountGenoHet(gds, target = "sample"))
\
# Alternative genotype count per marker head(getCountGenoAlt(gds, target = "marker")) # Alternative genotype count per sample head(getCountGenoAlt(gds, target = "sample"))
\
# Missing count per marker head(getCountGenoMissing(gds, target = "marker")) # Missing count per sample head(getCountGenoMissing(gds, target = "sample"))
\
# Reference allele count per marker head(getCountAlleleRef(gds, target = "marker")) # Reference allele count per sample head(getCountAlleleRef(gds, target = "sample"))
\
# Alternative allele count per marker head(getCountAlleleAlt(gds, target = "marker")) # Alternative allele count per sample head(getCountAlleleAlt(gds, target = "sample"))
\
# Missing allele count per marker head(getCountAlleleMissing(gds, target = "marker")) # Missing allele count per sample head(getCountAlleleMissing(gds, target = "sample"))
\
# Reference read count per marker head(getCountReadRef(gds, target = "marker")) # Reference read count per sample head(getCountReadRef(gds, target = "sample"))
\
# Alternative read count per marker head(getCountReadAlt(gds, target = "marker")) # Alternative read count per sample head(getCountReadAlt(gds, target = "sample"))
\
# Sum of reference and alternative read counts per marker head(getCountRead(gds, target = "marker")) # Sum of reference and alternative read counts per sample head(getCountRead(gds, target = "sample"))
\
# Mean of reference allele read count per marker head(getMeanReadRef(gds, target = "marker")) # Mean of reference allele read count per sample head(getMeanReadRef(gds, target = "sample"))
\
# Mean of Alternative allele read count per marker head(getMeanReadAlt(gds, target = "marker")) # Mean of Alternative allele read count per sample head(getMeanReadAlt(gds, target = "sample"))
\
# SD of reference allele read count per marker head(getSDReadRef(gds, target = "marker")) # SD of reference allele read count per sample head(getSDReadRef(gds, target = "sample"))
\
# SD of Alternative allele read count per marker head(getSDReadAlt(gds, target = "marker")) # SD of Alternative allele read count per sample head(getSDReadAlt(gds, target = "sample"))
\
# Minor allele frequency per marker head(getMAF(gds, target = "marker")) # Minor allele frequency per sample head(getMAF(gds, target = "sample"))
\
# Minor allele count per marker head(getMAC(gds, target = "marker")) # Minor allele count per sample head(getMAC(gds, target = "sample"))
\
You can get the proportion of each genotype call with prop = TRUE
.
head(getCountGenoRef(gds, target = "marker", prop = TRUE)) head(getCountGenoHet(gds, target = "marker", prop = TRUE)) head(getCountGenoAlt(gds, target = "marker", prop = TRUE)) head(getCountGenoMissing(gds, target = "marker", prop = TRUE))
\
The proportion of each allele counts.
head(getCountAlleleRef(gds, target = "marker", prop = TRUE)) head(getCountAlleleAlt(gds, target = "marker", prop = TRUE)) head(getCountAlleleMissing(gds, target = "marker", prop = TRUE))
\
The proportion of each allele read counts.
head(getCountReadRef(gds, target = "marker", prop = TRUE)) head(getCountReadAlt(gds, target = "marker", prop = TRUE))
Based on the statistics we obtained, we can filter out less reliable markers and samples using setMarFilter()
and setSamFilter()
, respectively.
gds <- setMarFilter(gds, missing = 0.2, het = c(0.1, 0.9), maf = 0.05) gds <- setSamFilter(gds, missing = 0.8, het = c(0.25, 0.75))
\
setCallFilter()
is another type of filtering which works on each genotype call that is a data point at a marker in a sample. We can replace some genotype calls with missing based on the specified criteria. If you would like to filter out less reliable genotype calls that are only supported by less than 5 reads, set the arguments as below.
gds <- setCallFilter(gds, dp_count = c(5, Inf))
\
If needed to remove genotype calls supported by too many reads, which might be the results of mismapping from repetitive sequences, set as follows.
# Filtering genotype calls based on total read counts gds <- setCallFilter(gds, dp_qtile = c(0, 0.9)) # Filtering genotype calls based on reference read counts # and alternative read counts separately. gds <- setCallFilter(gds, ref_qtile = c(0, 0.9), alt_qtile = c(0, 0.9))
\
Usually reference reads and alternative reads show different data distributions. Thus, we can set the different thresholds for them via dp_qtile
, ref_qtile
, and alt_qtile
to filter out genotype calls based on quantiles of total, reference, and alternative read counts in each sample.
\
Here, the following codes filter out calls supported by less than 5 reads and then filter out markers showing a missing rate of more than 20%.
gds <- setCallFilter(gds, dp_count = c(5, Inf)) gds <- setMarFilter(gds, missing = 0.2)
\
Based on our study using simulation data and real data for a rice F2 population derived from a cross between distant relatives (cultivar x wild species), we recommend the setting of ref_qtile = c(0, 0.9), alt_qtile = c(0, 0.9)
to filter out markers with over represented reads. If your population contains samples that have only either of reference or alternative reads at the majority of markers, filtering with ref_qtile = c(0, 0.9), alt_qtile = c(0, 0.9)
will set missing to a large portion of markers for the samples. In that case, it is better to set dp_qtile = c(0, 0.9)
. In addition, the error correction by GBScleanR does not require any filtering for markers based on missing rate, heterozygosity, and allele frequency. Therefore, setMarFilter()
and setSamFilter()
will be used only when you have specific markers and samples that should be removed. In that case, please specify marker IDs and sample IDs to the id
argument of setMarFilter()
and setSamFilter()
, respectively.
gds <- setCallFilter(gds, ref_qtile = c(0, 0.9), alt_qtile = c(0, 0.9)) invalid_mar <- getMarID(gds)[1:5] gds <- setMarFilter(gds, id = invalid_mar) invalid_sam <- getSamID(gds)[1:3] gds <- setSamFilter(gds, id = invalid_sam)
In addition to those statistics based filtering functions, r Biocpkg("GBScleanR")
provides a filtering function based on relative marker positions. Markers locating too close each other usually have redundant information, especially if those markers are closer each other than the read length, in which case the markers are supported by completely (or almost) the same set of reads. To select only one marker from those markers, we can sue thinMarker()
. This function selects one marker having the least missing rate from each stretch of the specified length. If some markers have the least missing rate, select the first marker in the stretch.
# Here we select only one marker from each 150 bp stretch. gds <- thinMarker(gds, range = 150)
\
We can obtain the summary statistics using countGenotype()
, countRead()
, and calcReadStats()
for only the SNPs and samples retained after the filtering. Importantly, we need to set node = "filt
if we have apllied setCallFiler()
. Otherwise, countGenotype()
used the raw genotype calls.
gds <- countGenotype(gds, node = "filt") gds <- countRead(gds, node = "filt")
\
We can check which markers and samples are retained after the filtering using validSnp()
and validSam()
.
head(validMar(gds)) head(validSam(gds))
\
The class methods of GbsrGenotypeData
basically work with only the markers and samples retained after filtering. To use all markers and samples, please specify valid = FALSE
to the GbsrGenotypeData
class methods.
nmar(gds) nmar(gds, valid = FALSE)
\
We can reset filtering as following.
# Reset the filter on markers gds <- resetMarFilter(gds) # Reset the filter on samples gds <- resetSamFilter(gds) # Reset the filter on calls gds <- resetCallFilter(gds) # Reset all filters gds <- resetFilter(gds)
The error correction algorithm of r Biocpkg("GBScleanR")
bases on the HMM assuming observed allele read counts for each SNP marker along a chromosome as the outputs from a sequence of latent true genotypes. Our model supposes that a population of $N^o \geq 1$ sampled offspring was originally derived form the crosses between $N^f \geq 2$ parent individuals. The parents can be inbred lines having homozygotes at all markers and outbred lines in which markers show heterozygous genotype.
The update on Mar 7, 2024 added a function to set sample replicate information
to jointly evaluate read counts for replicates in the genotype estimation by
the estGeno()
function. The estGeno()
function sums up the read counts of
replicates specified by setReplicates()
and estimates genotypes based on the
summed-up read counts. The samples specified as replicates in setReplicates()
will have the same genotypes at all markers in the estimated genotypes obtained
via estGeno()
. The setReplicates()
function assumes that replicate
information would be supplied for all samples in the data including parents via
the replicates
argument. In addition, the setReplicates()
function assumes
that the samples that are assigned same numbers or characters via the
replicates
argument are replicates. Therefore, the ordering of samples in the
data and the identifiers in the vector specified to replicates
should match.
Replicates can be specified as follows.
First, it is better to confirm the ordering of samples in the data with the
setting "valid = FALSE" to obtain all sample IDs.
sample_id <- getSamID(gds, valid = FALSE) sample_id
Here, as an example, we assume that the samples at odd indices in the
sample_id
vector are the replicates of the next samples at even indices. For
example, F2_1054 and F2_1055 are replicates for which DNA samples were extracted
from the same F2 individual although they have different samples IDs. In this
case, you can set replicate information as shown below.
gds <- setReplicates(gds, replicates = rep(1:51, each = 2))
As another example, if parents in the data are Founder1 and Founder2 and
replicates are F2_1054 and F2_1022 for Founder1 and F2_1178 and F2_1637 for
Founder2, you should give a vector to the replicates
argument like the
following.
rep_of_parent1 <- sample_id %in% c("Founder1", "F2_1054", "F2_1022") rep_of_parent2 <- sample_id %in% c("Founder2", "F2_1178", "F2_1637") sample_id[rep_of_parent1] <- "Founder1" sample_id[rep_of_parent2] <- "Founder2" gds <- setReplicates(gds, replicates = sample_id)
If you set replicates for parents, you should give a sample id of the replicates
as an identifier for a parent in setParents()
as described in the next
section. If you set replicates for parents after setting parents by
setParents()
, the replicates for parents will be also set as parents with
assigning the same member ID for the replicates of each parent.
To reset the assigned replicate information, please use setReplicates()
with
specifying different values to the replicates
argument.
gds <- setReplicates(gds, replicates = seq_len(nsam(gds, valid = FALSE)))
As the first step for genotype error correction, we have to specify which samples are the parents of the population via setParents()
. In the case of genotype data in the biparental population, people usually filter out SNPs which are not monomorphic in each parental sample and not biallelic between parents. setParents()
automatically do this filtering, if you set mono = TRUE
and bi = TRUE
.
p1 <- grep("Founder1", getSamID(gds, valid = FALSE), value = TRUE) p2 <- grep("Founder2", getSamID(gds, valid = FALSE), value = TRUE) gds <- setParents(gds, parents = c(p1, p2), mono = TRUE, bi = TRUE)
If you set replicates for parents, you should give a sample id of the replicates
as an identifier for a parent in setParents()
. In the last example in the
previous section, we set three replicates for each parent. To properly set
parents, we should specify either of "Founder1", "F2_1054", and "F2_1022" for
p1
and either of "Founder2", "F2_1178", "F2_1637" for p2
.
The next step is to visualize statistical summaries of the data. Get genotype data summaries as mentioned in the previous section.
gds <- countGenotype(gds)
\
Then, get histograms.
histGBSR(gds, stats = "missing")
\
histGBSR(gds, stats = "het")
\
histGBSR(gds, stats = "raf")
\
As the histograms showed, the data contains a lot of missing genotype calls with unreasonable heterozygosity in a F2 population. Reference allele frequency shows a bias to reference allele. If you can say your population has no strong segregation distortion in any positions of the genome, you can filter out the markers having too high or too low reference allele frequency.
# filter out markers with reference allele frequency # less than 5% or more than 95%. gds <- setMarFilter(gds, maf = 0.05)
\
However, sometimes filtering based on allele frequency per marker removes all markers from regions truly showing segregation distortion. Although heterozygosity can be a criterion to filter out markers, this will removes too many markers which even contains useful information for genotyping. In addition, as we described in the previous section, the error correction by GBScleanR does not require any filtering for markers based on missing rate, heterozygosity, and allele frequency. \
If we found poor quality samples in you dataset based on missing rate, heterozygosity, and reference allele frequency, we can omit those samples with setSamFilter()
.
# Filter out samples with more than 90% missing genotype calls, # less than 5% heterozygosity, and less than 5% minor allele frequency. gds <- setSamFilter(gds, missing = 0.9, het = c(0.05, 1), maf = 0.05)
\
Before filtering using setMarFilter()
and setSamFilter()
, we recomend that you conduct filtering on each genotype call based on read depth. The error correction via r Biocpkg("GBScleanR")
is robust against low coverage calls, while genotype calls messed up by mismapping might lead less reliable error correction. Therefore, filtering for extremely high coverage calls are necessary rather than that for low coverage ones.
# Filter out genotype calls supported by reads less than 2 reads. gds <- setCallFilter(gds, dp_count = c(2, Inf)) # Filter out genotype calls supported by reads more than 100. gds <- setCallFilter(gds, dp_count = c(0, 100)) # Filter out genotype calls based on quantile values # of read counts at markers in each sample. gds <- setCallFilter(gds, ref_qtile = c(0, 0.9), alt_qtile = c(0, 0.9))
\
Since missing genotype calls left in the data basically give no negative effect on genotype error correction. Therefore, you can leave any missing genotype calls. We can, however, remove markers based on missing genotype calls.
# Remove markers having more than 75% of missing genotype calls gds <- setMarFilter(gds, missing = 0.2) nmar(gds)
\
To check statistical summaries of the filtered genotype data, we need to set node = "filt
. Otherwise, countGenotype()
used the raw genotype data.
gds <- countGenotype(gds, node = "filt")
\
histGBSR(gds, stats = "missing")
\
histGBSR(gds, stats = "het")
\
histGBSR(gds, stats = "raf")
\
We can still see the markers showing distortion in allele frequency, while the expected allele frequency is 0.5 in a F2 population. To investigate that those markers having distorted allele frequency were derived from truly distorted regions or just error prone markers, we must check if there are regions where the markers with distorted allele frequency are clustered.
plotGBSR(gds, stats = "raf")
\
No region seem to have severe distortion. Based on the histogram of reference allele frequency, we can roughly cut off the markers with frequency more than 0.75 or less than 0.25, in other words, less than 0.25 of minor allele frequency. As we mentioned already in the previous section, the error correction by GBScleanR basically works finely without any filtering for markers based on missing rate, heterozygosity, and allele frequency.
gds <- setMarFilter(gds, maf = 0.25) nmar(gds)
\
Let's see the statistics again.
gds <- countGenotype(gds) histGBSR(gds, stats = "missing")
\
histGBSR(gds, stats = "het")
\
histGBSR(gds, stats = "raf")
\
At the end of filtering, check marker density and genotype ratio per marker along chromosomes.
# Marker density plotGBSR(gds, stats = "marker")
\
plotGBSR(gds, stats = "geno")
\
The coord
argument controls the number of rows and columns of the facets in the plot.
Before executing the function for true genotype estimation, we need to build a scheme object. The update on May 17, 2024 added a wrapper function for scheme information preparation. If your population has been developed in a relatively simple scheme, you can use the makeScheme()
function.
# For selfed F2 population gds <- makeScheme(gds, generation = 2, crosstype = "self") # For sibling-crossed F2 population gds <- makeScheme(gds, generation = 2, crosstype = "sib") # For selfed F5 population gds <- makeScheme(gds, generation = 5, crosstype = "self") # For F1 population gds <- makeScheme(gds, generation = 1) # the crosstype argument will be ignored.
When your population has $2^n$ parents specified by setParents()
, makeScheme()
assumes those parents were crossed in the "funnel" design in which $2^n$ parents are crossed to obtain $2^n/2$ F1 hybrids followed by successive intercrossings (pairings) of the hybrids to combine the genomes of all parents in one family of siblings. The makeScheme()
function assumes that the parents that were assigned an odd number member ID (N) in setParents()
had been crossed with the parent that were assigned an even number (N+1). For example, if you set parents as shown below. The makeScheme()
function prepare a scheme information that indicates the intercrossings of "p1 x p2", "p3 x p4", "p5 x p6", and "p7 x p8" followed by crossing of "p1xp2_F1 x p3xp4_F1" and "p5xp6_F1 x p7xp8_F1" and then crossing of the two 4-way crossed liens to produce 8-way crossed hybrid lines. If, for example, generation = 5
indicating an F5 generation was specified to makeScheme()
, the function adds 4 successive selfing or sibling crossings in the scheme.
# Do not run. gds <- setParents(gds, parents = c("p1", "p2", "p3", "p4", "p5", "p6", "p7", "p8")) # Member IDs will be 1, 2, 3, 4, 5, 6, 7, and 8 # for p1, p2, p3, p4, p5, p6, p7, and p8, respectively.
In many cases, makeScheme()
is enough to prepare scheme information. However, if your population underwent more complicated crossings, please register scheme information step-by-step as shown below.
Our sample data is of a biparental F2 population derived from inbred parents. Therefore, we should run initScheme()
and addScheme()
as following.
# As always the first step of breeding scheme would be "pairing" cross(es) of # parents, never be "selfing" and a "sibling" cross, # the argument `crosstype` in initScheme() was deprecated on the update on April 6, 2023. # gds <- initScheme(gds, crosstype = "pairing", mating = matrix(1:2, 2)) gds <- initScheme(gds, mating = rbind(1, 2)) gds <- addScheme(gds, crosstype = "selfing")
\
The function initScheme()
initializes the scheme object with information about parents. You need to specify a matrix indicating combinations of mating
, in which each column shows a pair of parental samples. For example, if you have only two parents, the mating
matrix should be mating = matrix(1:2, nrow = 2, ncol = 1)
or equivalents. The indices used in the matrix should match with the IDs labeled to parental samples by setParents()
. To confirm the IDs for parental samples, run the following code.
getParents(gds)
The created GbsrScheme
object is set in the scheme
slot of the GbsrGenotypeData
object.
The function addScheme()
adds the information about the next breeding step of your population. In the case of our example data, the second step was selfing to produce F2 individuals from the F1 obtained via the first parent crossing.
\
The codes shown below in the rest of the section "Prepare scheme information" are sample codes assuming some specific situations that are not applicable for the sample data used in this vignette. Therefore, you will get error messages if you run the codes.
If your population was derived from a 4-way or 8-way cross, you need to add more paring
steps. In the case of 8-way RILs developed by three pairing crosses followed by five selfing cycles, the scheme object should be built as following. First we need to initialize the scheme object with specifying the first mating scheme. The crosstype argument should be "pairing" and the mating argument should be given as a matrix in which each pairing combination of parents are shown in each column. The following case indicates the pairing of parent 1 and 2 as well as 3 and 4, 5 and 6, and 7 and 8.
# As always the first step of breeding scheme would be "pairing" cross(es) of # parents, never be "selfing" and a "sibling" cross, # the argument `crosstype` in initScheme() was deprecated on the update on April 6, 2023. # gds <- initScheme(gds, crosstype = "pair", mating = cbind(c(1:2), c(3:4), c(5:6), c(7:8))) # Do not run. gds <- initScheme(gds, mating = cbind(c(1:2), c(3:4), c(5:6), c(7:8)))
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Now the progenies of the crosses above have member ID 9, 10, 11, and 12 for each combination of mating. You can check IDs with showScheme().
Then, add the step to make 4-way crosses.
# Do not run. gds <- addScheme(gds, crosstype = "pair", mating = cbind(c(9:10), c(11:12))) # Check IDs. showScheme(gds)
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Add the last generation of the paring step .
# Do not run. gds <- addScheme(gds, crosstype = "pair", mating = cbind(c(13:14))) #' # Check IDs. showScheme(gds)
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Now we have the scheme information of a 8-way cross.
The follwoing steps add the selfing cycles.
# Inbreeding by five times selfing. # Do not run. gds <- addScheme(gds, crosstype = "self") gds <- addScheme(gds, crosstype = "self") gds <- addScheme(gds, crosstype = "self") gds <- addScheme(gds, crosstype = "self") gds <- addScheme(gds, crosstype = "self")
You can set crosstype = "sibling"
or crosstype = "random"
, if your population was developed through sibling crosses or random crosses, respectively.
The update on April 4, 2023 introduced new function to GBScleanR. The genotype estimation algorithm in estGeno() supports populations that consist of samples belonging to different pedigree. For example, if you have a population of F1 samples that derived from three different crosses of four parents: Founder1 x Founder2, Founder1 x Founder3, Founder1 x Founder4. You can build a scheme info as following.
# Do not run. gds <- setParents(object = gds, parents = c("Founder1", "Founder2", "Founder3", "Founder4")) gds <- initScheme(object = gds, mating = cbind(c(1, 2), c(1, 3), c(1,4))) # The initScheme() function here automatically set 5, 6, and 7 as member ID to # the progenies of the above maiting (pairing) combinations, respectively. # Then you have to assign member IDs to your samples to indicate which sample # belongs to which pedigree. gds <- assignScheme(object = gds, id = c(rep(5, 10), rep(6, 15), rep(7, 20)))
The assignScheme() assign member IDs id
to the samples in order.
Please confirm the order of the member IDs in id
and the order of the
sample IDs shown by getSamID(gds).
# Do not run. # Get sample ID sample_id <- getSamID(object = gds) # Initialize the id vector id <- integer(nsam(gds)) # Assume your samples were named with prefixes that indicate which # sample was derived from which combination of parents. id[grepl("P1xP2", sample_id)] <- 5 id[grepl("P1xP3", sample_id)] <- 6 id[grepl("P1xP4", sample_id)] <- 7 gds <- assignScheme(object = gds, id = id)
The following codes suppose that you built the scheme object for the example data that is a biparental F2 population derived from a cross between inbred parents, not for the 8-way RILs explained above.
Now we can execute genotype estimation for error correction. r Biocpkg("GBScleanR")
estimates error pattern via iterative optimization of parameters for genotype estimation. We could not guess the best number of iterations, but our simulation tests showed iter = 4
usually saturates the improvement of estimation accuracy.
gds <- estGeno(gds, iter = 4)
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If your population derived from outbred parents, please set het_parents = TRUE
.
# Do nut run # This is an example to show the case to use "het_parents = TRUE". gds <- estGeno(gds, het_parent = TRUE, iter = 4)
The larger number of iterations makes running time longer. If you would like to execute no optimization, set optim = FALSE
or iter = 1
.
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# Following codes do the same. # Do nut run # These are examples to show the case to set "iter = 1" or "optim = FALSE". gds <- estGeno(gds, iter = 1) gds <- estGeno(gds, optim = FALSE)
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All of the results of estimation are stored in the gds file linked to the GbsrGenotypeData
object. You can obtain the estimated genotype data via the getGenotype()
function with node = "cor"
.
est_geno <- getGenotype(gds, node = "cor")
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r Biocpkg("GBScleanR")
also estimates phased parent genotypes and you can access it.
parent_geno <- getGenotype(gds, node = "parents")
r Biocpkg("GBScleanR")
first internally estimate phased haplotype and then convert them to genotype calls. If you need the estimated haplotype data, run getHaplotype()
.
est_hap <- getHaplotype(gds)
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The function gbsrGDS2VCF()
generate a VCF file containing the estimated genotype data and phased haplotype information. The estimated haplotypes are indicated in the FORMAT field with the HAP tag. The parent genotypes correspond to each haplotype are indicated in the INFO field with the PGT tag. HAP shows the pair of haplotype for each marker of each sample, while PGT shows the allele of each haplotype. HAP is indicated by two numbers separated by a pipe symbol "|". Each of the numbers takes one of the numbers from 1 to 2N, where N is the number of parents.
PGT is indicated by 2N numbers separated by a pipe symbol "|". The first number in PGT represents the allele of haplotype 1 at the marker, and the rest numbers also show the alleles of rest haplotypes 2, 3 and 4, if your population is of biparental diploid samples. As a default, gbsrGDS2VCF()
outputs the estimated genotype data as entries of the CGT tag in the FORMAT field. With node = "cor"
, you can output a VCF file in which GT field was replaced with the estimated genotype data obtained via estGeno()
.
out_fn <- tempfile("sample_est", fileext = ".vcf.gz") gbsrGDS2VCF(gds, out_fn) gbsrGDS2VCF(gds, out_fn, node = "cor")
The output vcf file contains data like the one shown below.
out <- system2("zcat", paste(out_fn, "head -n 15", sep = "|"), stdout = TRUE) gsub("\t", " ", out)
In the output vcf, "PGT=0|0|1|1" in the INFO field indicates the parental
samples alleles for haplotype 1, 2, 3, and 4 separated by |. The numbers 0 and 1 represent reference and alternative alleles that are A and G at the marker shown above, resectively.
FAD and FGT in the FORMAT field show the allele read counts and genotype call after filtering by setCallFiler()
.
HAP and EDS indicate descendent haplotypes and haplotype dosage at the given marker. EDS will be provided only if you specified two parents in setParents()
and specified het_parent = FALSE
for estGeno()
.
Alternatively, you can also output the genotype data into a CSV file using gbsrGDS2CSV()
out_fn <- tempfile("sample_est", fileext = ".csv") gbsrGDS2CSV(gds, out_fn) gbsrGDS2CSV(gds, out_fn, node = "cor")
You can format the output for the r CRANpkg("qtl")
package.
out_fn <- tempfile("sample_est", fileext = ".csv") gbsrGDS2CSV(gds, out_fn, format = "qtl") gbsrGDS2CSV(gds, out_fn, node = "cor", format = "qtl")
When you set format = "qtl"
, the marker positions will be automatically converted from physical positions (bp) to genetic distances (cM). The conversion is performed by multiplying the physical positions by the value set to bp2cm
. The default is bp2cm = 4e-06
if format = "qtl"
.
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Please use reopenGDS()
to open the connection again if you need.
gds <- reopenGDS(gds)
To safely close the connection to the GDS file, use closeGDS()
.
closeGDS(gds)
sessionInfo()
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