plot_trimming: Plot trimming process

In ucl-cssb/flopr: A package for the normalisation and calibration of flow cytometry and plate reader spectrophotometry data

Plot trimming process

Description

Plot trimming process

Usage

plot_trimming(
  flow_frame,
  bacteria_flow_frame,
  singlet_flow_frame,
  normalised_flow_frame,
  calibrated_flow_frame,
  flu_channels,
  out_name,
  normalised,
  calibrate
)

Arguments

flow_frame	the original flowFrame
bacteria_flow_frame	the flowFrame with debris removed
singlet_flow_frame	the flowFrame with debris and doublets removed
normalised_flow_frame	the normalised flowFrame
calibrated_flow_frame	the calibrated flowFrame with debris and doublets removed
flu_channels	a vector of strings of the fluorescence channels to keep in the processed data and plotting. Defaults to "BL1-H".
out_name	the filename for the outputted flowFrame
normalised	a Boolean flag to determine whether to normalise
calibrate	a Boolean flag to determine whether to convert fluorescence to MEF values. Requires an .fcs file with named "*beads*.fcs". Defaults to FALSE.

ucl-cssb/flopr documentation built on Feb. 2, 2025, 10:30 p.m.