fluoroClust: A function to cluster flow cytometry data by one fluorescence...

Description Usage Arguments Value

View source: R/fluoroClust.R

Description

Cluster data in a flowFrame using the flowClust package. Given a fluorescence channel and, optionally, prior values for the on and off peak locations, determines how many clusters there are and produces information on the populations.

Usage

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fluoroClust(fF, clust.type = "ICL", channel = "BL1-H",
  crude.threshold = 3.5, do.plot = F)

Arguments

fF

a flowFrame to cluster.

clust.type

choose a clustering method; "ICL" or "crude".

channel

the fluorescence channel on which to cluster.

crude.threshold

the threshold to use if the "crude" method is chosen.

do.plot

a Boolean flag to determine whether to produce plots showing the trimming of each flowFrame. Defaults to FALSE.

Value

a data.frame containing num_samples, the number of samples clustered, max_clust_mean, the mean value of fluoresence in the "on" cluster, and max_clust_prop, the proportion of the total population that is "on".


ucl-cssb/fluoroClust documentation built on May 3, 2019, 2:22 p.m.