read.smartseq2.bams: read.smartseq2.bams

Description Usage Arguments Value

View source: R/momentum_routines.R

Description

DEPRECATED: Read in cell-specific bam files for SMART-seq2 measurement This function is deprecated. Please use velocyto.py to prepare loom file from SMART-seq2 bam files.

Usage

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read.smartseq2.bams(bam.files, annotation.file, min.exon.count = 100,
  n.cores = defaultNCores())

Arguments

bam.files

list of bam files

annotation.file

refFlat genome annotation file (use gtfToGenePred to generate refFlat file from gtf)

min.exon.count

minimum number of reads (across all cells) for an exon to be considered expressed in the dataset

n.cores

number of cores to use

Value

a list containing: emat - exonic (spliced) read count matrix ; iomat - intronic (unspliced) matrix; smat - spanning read matrix; base.df - data frame containing gene structural information; exons - exon annotation and read counts; genes - gene annotation table with additional structural info; expr.lstat - gene length statistics when considering only expressed exons


velocyto-team/velocyto.R documentation built on Dec. 8, 2019, 3:07 a.m.